CH391L/S13/In vitro Selection of FNAs
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Introduction
Types of Functional Nucleic Acids
In vitro Selection of Functional Nucleic Acids
Ribozymes
Deoxyribozymes
Aptamers
Extra
Oligonucleotides are chemically synthesized from DNA phosphoramidite monomers. Briefly, activated phosphoramidite monomers are added in the 3' to 5' direction using a cyclical activation and blocking chemistry to obtain a DNA polymer linked by phosphodiester bonds.
Chemical synthesis is currently limited to oligonucleotides of about 200 nt in length.
