CHE.496/2008/Projects/2/Group 2: Difference between revisions
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**Biologically-produced plastics represent a sustainable chemical industry that is not dependent on petroleum | **Biologically-produced plastics represent a sustainable chemical industry that is not dependent on petroleum | ||
**Three genes necessary for PHB synthesis: phaA, phaB and phaC (from Pohlmann ''et al.'', 2006) | **Three genes necessary for PHB synthesis: phaA, phaB and phaC (from Pohlmann ''et al.'', 2006) | ||
**phaA (acetyl-CoA acetyltransferase) [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NC_008313.1&from=1559207&to=1560388&dopt=fasta Nucleotide sequence] | **Pathway: 2 acetyl-CoA are condensed into acetoacetyl-CoA by phaA. Then, phaB (NADPH-dependent) reduces this to (R)-3-hydroxybutyryl-CoA. Finally, this is polymerized into PHB by phaC. | ||
**''phaA'' (code for acetyl-CoA acetyltransferase) [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NC_008313.1&from=1559207&to=1560388&dopt=fasta Nucleotide sequence] | |||
**Possible use in tissue engineering applications | **Possible use in tissue engineering applications | ||
*Biological degradation of plastic | *Biological degradation of plastic | ||
**PHAs are biodegradable. | **PHAs are biodegradable. |
Revision as of 11:23, 28 March 2008
Group 2's project overview and part design
List of initial ideas
- Foundational
- Parts for genetic circuits
- New chassis (environment, etc.)
- Optimizing existing parts (e.g., inverter)
- Light sensor
- Compartmentalization/insulation
- Molecular screens (filters) that enable signal isolation and insulation (inside the cells and in between cells)
- Computational
- modeling of biotech-relevant microorganisms
- Synthetic Biology Tools
- Quality characterization techniques
- Ways to insulate biological signals
- Metabolic Engineering
- Polysaccharide synthesis (for food)
- Bioplastic production
- Chemical degradation (toxin breakdown)
- Oil
- CO2
- Plastics
- Genetic Circuit Engineering
- Light-based repressilator
- Light switch
- Biosensing
- Ethylene biosensor
- Algae that sense light and displays permanent memory
List of top two project ideas
- Biological production of plastic
- Polyhydroxyalkanoates (PHAs) are naturally synthesized polyesters in Ralstonia eutropha
- These PHAs are used for energy storage
- Polyhydroxybutyrate (PHB) is a PHA and could possible replace polypropylene
- Biologically-produced plastics represent a sustainable chemical industry that is not dependent on petroleum
- Three genes necessary for PHB synthesis: phaA, phaB and phaC (from Pohlmann et al., 2006)
- Pathway: 2 acetyl-CoA are condensed into acetoacetyl-CoA by phaA. Then, phaB (NADPH-dependent) reduces this to (R)-3-hydroxybutyryl-CoA. Finally, this is polymerized into PHB by phaC.
- phaA (code for acetyl-CoA acetyltransferase) Nucleotide sequence
- Possible use in tissue engineering applications
- Biological degradation of plastic
- PHAs are biodegradable.