CHE.496/2008/Schedule/Genetic circuit engineering

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(Genetic circuit engineering (Part 1))
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*'''A synthetic oscillatory network of transcriptional regulators [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html link]
*'''A synthetic oscillatory network of transcriptional regulators [http://www.nature.com/nature/journal/v403/n6767/abs/403335a0.html link]
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**This article by Elowitz is one of the ‘poster boys’ of synthetic biology. This is for several reasons. The first is that it was incorporated into standardized biological parts. The second is that it was very successfully modeled using a fluorescent tag at the end of each cycle. Third is the novel idea of this system. Rather than using some kind of circadian or natural rhythm, Elowitz was able to induce a completely man-made, chemical oscillating system within E. coli. Also, this oscillation occurs at a speed slower than that of natural cell division, and therefore the cell must transmit the cycle to the offspring.
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*'''Construction of a genetic toggle switch in ''Escherichia coli'' [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html link]
*'''Construction of a genetic toggle switch in ''Escherichia coli'' [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html link]
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**This article discusses a bistable switch incorporated into E. coli. Like the repressilator created by Elowitz, this switch involves circuit engineering. This switch was successful in that it could be controlled between its two states. A bistable switch which randomly switches is useless. A bistable switch induced by thermal or chemical signals is very useful in biotechnology applications. For example, if a precursor was needed for a product, all the cells in the broth could produce this precursor. With a chemical or thermal signal, all the cells could then switch to producing the end product from this precursor.
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==Genetic circuit engineering (Part 2)==
==Genetic circuit engineering (Part 2)==
*'''Discussion leader: Dan
*'''Discussion leader: Dan

Revision as of 16:09, 27 February 2008

CHE.496: Biological Systems Design Seminar

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Genetic circuit engineering (Part 1)

  • Discussion leader: Kevin


  • A synthetic oscillatory network of transcriptional regulators link
    • This article by Elowitz is one of the ‘poster boys’ of synthetic biology. This is for several reasons. The first is that it was incorporated into standardized biological parts. The second is that it was very successfully modeled using a fluorescent tag at the end of each cycle. Third is the novel idea of this system. Rather than using some kind of circadian or natural rhythm, Elowitz was able to induce a completely man-made, chemical oscillating system within E. coli. Also, this oscillation occurs at a speed slower than that of natural cell division, and therefore the cell must transmit the cycle to the offspring.


  • Construction of a genetic toggle switch in Escherichia coli link
    • This article discusses a bistable switch incorporated into E. coli. Like the repressilator created by Elowitz, this switch involves circuit engineering. This switch was successful in that it could be controlled between its two states. A bistable switch which randomly switches is useless. A bistable switch induced by thermal or chemical signals is very useful in biotechnology applications. For example, if a precursor was needed for a product, all the cells in the broth could produce this precursor. With a chemical or thermal signal, all the cells could then switch to producing the end product from this precursor.


Genetic circuit engineering (Part 2)

  • Discussion leader: Dan


  • Environmentally controlled invasion of cancer cells by engineered bacteria link


  • Environmental signal integration by a modular AND gate link
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