CHE.496/2008/Schedule/Natural biological parts
From OpenWetWare
Natural biological parts
- Intro to Gene Designer (George)
- Discussion leader: Patrick
- Another side of genomics: Synthetic biology as a means for the exploitation of whole-genome sequence information link
- Purpose: The paper reviews the area of Synthetic Biology – the design/development of biosystems founded on the synthesis of DNA as well as recent developments in the field.
- Background material is given in the various fields and technologies (synthetic genes, DNA recombination tech, PCR and so on).
- The process for creating a synthetic gene: target DNA is produced by programmed chemical synthesis of short oligonucleotides where longer DNA molecules are formed together via ligation, PCR, and doing either of the following:
- Synthesizing the complete sequence and ligating the backbone
- Synthesizing part of the sequence, leaving gaps in the fragile hybrid and filling in the gaps with DNA polymerase enzymes
- In situ microarray synthesis represents a cost-effective technology that can combine pieces of DNA to form larger pieces of DNA in a cheaper and faster manner than older technologies.
- Purpose: The paper reviews the area of Synthetic Biology – the design/development of biosystems founded on the synthesis of DNA as well as recent developments in the field.
- Codon bias and heterologous protein expression link
Exercise:
