CRI Genomics Core:Protocols

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==Microarray==
==Microarray==
[[RNA Quality Control|RNA QC]]
[[RNA Quality Control|RNA QC]]
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<showhide>'''[[Affymetrix |Affymetrix]]'''__HIDER__
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<hide>
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'''[[Affymetrix |Affymetrix]]'''
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{{hide|1=
:* [[Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing|One cycle labeling]]
:* [[Affymetrix One Cycle Eukaryotic Gene Expression Sample Processing|One cycle labeling]]
:* [[Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing|Small sample labeling]]
:* [[Affymetrix Two Cycle Eukaryotic Gene Expression Sample Processing|Small sample labeling]]
:* [[Affymetrix Prokaryotic Gene Expression Sample Processing|Prokaryotic Labeling]]
:* [[Affymetrix Prokaryotic Gene Expression Sample Processing|Prokaryotic Labeling]]
:* [[Affymetrix DNA labelling for gene expression arrays|DNA Labeling]]
:* [[Affymetrix DNA labelling for gene expression arrays|DNA Labeling]]
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</hide></showhide>
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}}
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<showhide>'''[[Illumina]]'''__HIDER__
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<hide>
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'''[[Illumina]]'''
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{{hide|1=
:* [[CRI Illumina Eukaryotic Gene Expression Sample Processing|Expression Labeling]]
:* [[CRI Illumina Eukaryotic Gene Expression Sample Processing|Expression Labeling]]
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</hide></showhide>
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}}
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==Nucleic acid prep==
==Nucleic acid prep==
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[[CRI Plasmid prep |Plasmid]]
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[[CRI plasmid miniprep]]
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[[CRI minipreps in 96well plates]]
==Sequencing==
==Sequencing==
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[[CRI DNA sequencing|DNA sequencing]]
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[[CRI DNA sequencing and troubleshooting guide]]
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[[CRI plasmid sequencing reaction]]
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[[CRI BAC prep and sequencing reaction]]
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[[CRI PCR product sequencing]]
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[[CRI ethanol EDTA precipitation]]
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==General==
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[[CRI nanodrop users guide]]
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[[CRI colony PCR]]
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[[CRI cosmid prep]]
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[[CRI Sigma 4K15 centrifuge]]
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[[CRI DNA quantification on the tecan genios plate reader]]
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[[CRI using the genetix Qfill2]]
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[[CRI using the Jenway pH Meter 3310]]
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[[CRI eGel96]]

Current revision

CRUK logo CRI logo



Contents

Microarray

RNA QC

Affymetrix

Illumina

Nucleic acid prep

CRI plasmid miniprep

CRI minipreps in 96well plates


Sequencing

CRI DNA sequencing and troubleshooting guide

CRI plasmid sequencing reaction

CRI BAC prep and sequencing reaction

CRI PCR product sequencing

CRI ethanol EDTA precipitation

General

CRI nanodrop users guide

CRI colony PCR

CRI cosmid prep

CRI Sigma 4K15 centrifuge

CRI DNA quantification on the tecan genios plate reader

CRI using the genetix Qfill2

CRI using the Jenway pH Meter 3310

CRI eGel96

Personal tools