CTR:Notebook/Migrant/2016/07/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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**BioRuptor NGS: 10 cycles of 15 seconds on, 90 seconds off, High Power | **BioRuptor NGS: 10 cycles of 15 seconds on, 90 seconds off, High Power | ||
**Combined both tubes of sheared DNA and ran on a gel | **Combined both tubes of sheared DNA and ran on a gel | ||
[[ | [[Image:AMKE sheared07222016.jpg|60px]] | ||
1) 2uL 100bp low scale ladder, | 1) 2uL 100bp low scale ladder, | ||
3) 2uL AMKE P1 sheared DNA | 3) 2uL AMKE P1 sheared DNA | ||
Line 102: | Line 102: | ||
****65°C for 75 seconds | ****65°C for 75 seconds | ||
***65°C for 5 minutes | ***65°C for 5 minutes | ||
[[ | [[Image:AMKE TestPCR07252016.jpg|60px]] | ||
1) 2uL 100bp low scale ladder | 1) 2uL 100bp low scale ladder | ||
2) 2uL AMKE P1 PCR product | 2) 2uL AMKE P1 PCR product | ||
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**PCR cycle (NEBFINPC on JOHN Block B): | **PCR cycle (NEBFINPC on JOHN Block B): | ||
***98°C for 30 seconds | ***98°C for 30 seconds | ||
*** | ***10 cycles of: | ||
****98°C for 10 seconds | ****98°C for 10 seconds | ||
****65°C for 75 seconds | ****65°C for 75 seconds | ||
***65°C for 5 minutes | ***65°C for 5 minutes | ||
[[ | [[Image:FinalPCR.jpg|60px]] | ||
1) 2uL 100bp low scale ladder | 1) 2uL 100bp low scale ladder | ||
2) 2uL AMKE P1 PCR product | 2) 2uL AMKE P1 PCR product | ||
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***Elute in 30 uL LowTE | ***Elute in 30 uL LowTE | ||
*Qubit: | *Qubit: 7.68 ng/μL | ||
*Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2016-07-26) | *Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2016-07-26) | ||
[[Image:Bioanalyzer AMKE p1.PNG|500px]] | |||
[[ | |||
*[ Bioanalyzer Results] | *[http://openwetware.org/images/c/c3/2100_expert_High_Sensitivity_DNA_Assay_DE72901273_2016-08-05_07-04-39_%281%29.pdf Bioanalyzer Results] |
Latest revision as of 01:50, 27 September 2017
Project name | Main project page Previous entry Next entry |
BestRAD Library Prep - AMKE Plate 1
1) 2uL 100bp low scale ladder, 3) 2uL AMKE P1 sheared DNA
1) 2uL 100bp low scale ladder 2) 2uL AMKE P1 PCR product
1) 2uL 100bp low scale ladder 2) 2uL AMKE P1 PCR product
|