CTR:Notebook/Migrant/2016/08/23: Difference between revisions
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* Sonication (2016-08-23) | * Sonication (2016-08-23) | ||
**BioRuptor NGS: 4 cycles of 30 seconds on, 90 seconds off, High Power | **BioRuptor NGS: 4 cycles of 30 seconds on, 90 seconds off, High Power | ||
[[ | [[Image:Wholegenomesheared 08232016.JPG|60px]] | ||
1) 2uL 100bp low scale ladder, | 1) 2uL 100bp low scale ladder, | ||
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* End Repair and Size Selection- edited to size select for 400bp (2016-08-24) | * End Repair and Size Selection- edited to size select for 400bp (2016-08-24) | ||
**Made a diluted bead mixture with the ratio of 109ul beads: 75ul dH<sub>2</sub>O and added 160 ul of mixture to each tube | **Made a diluted bead mixture with the ratio of 109ul beads: 75ul dH<sub>2</sub>O and added 160 ul of mixture to each tube | ||
[[ | [[http://openwetware.org/wiki/Image:Size_selected_08_24_2016.JPG|75px]] | ||
1) 2uL 100bp low scale ladder, | 1) 2uL 100bp low scale ladder, | ||
Revision as of 17:03, 24 August 2016
 Project name
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Whole Genome Sequencing PCR-Free Library Prep for TRBL
1) 2uL 100bp low scale ladder, 3) 2uL sheared TRBL
→Sheared DNA to ~400bp.
[[1]] 1) 2uL 100bp low scale ladder, 3) 2uL TRBL
1) 2uL 100bp low scale ladder, 3) 2uL TRBL
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![[1]](http://openwetware.org/wiki/Image:Size_selected_08_24_2016.JPG%7C75px){kind=link}