CTR:Notebook/Migrant/2017/06/20: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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**BioRuptor NGS: 10 cycles of 15 seconds on, 90 seconds off, High Power | **BioRuptor NGS: 10 cycles of 15 seconds on, 90 seconds off, High Power | ||
**Combined both tubes of sheared DNA and ran on a gel | **Combined both tubes of sheared DNA and ran on a gel | ||
[[ | [[Image:ZolaP4 Sheared 06212017.JPG|50px]] | ||
1) 2uL 100bp low scale ladder, | 1) 2uL 100bp low scale ladder, | ||
3) 2uL Silvereye sheared DNA | 3) 2uL Silvereye sheared DNA | ||
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****65°C for 75 seconds | ****65°C for 75 seconds | ||
***65°C for 5 minutes | ***65°C for 5 minutes | ||
[[ | [[Image:ZolaP4 TestPCR 06212017.JPG|60px]] | ||
1) 2uL 100bp low scale ladder | 1) 2uL 100bp low scale ladder | ||
2) 5uL Silvereye PCR product | 2) 5uL Silvereye PCR product | ||
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**PCR cycle (NEBFINPC on JOHN Block B): | **PCR cycle (NEBFINPC on JOHN Block B): | ||
***98°C for 30 seconds | ***98°C for 30 seconds | ||
***''' | ***'''11''' cycles of: | ||
****98°C for 10 seconds | ****98°C for 10 seconds | ||
****65°C for 75 seconds | ****65°C for 75 seconds | ||
***65°C for 5 minutes | ***65°C for 5 minutes | ||
[[ | [[Image:ZolaP4 FinalPCR 06212017.JPG|60px]] | ||
1) 2uL 100bp low scale ladder | 1) 2uL 100bp low scale ladder | ||
2) 5uL Silvereye PCR product | 2) 5uL Silvereye PCR product | ||
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***Elute in 30 uL LowTE | ***Elute in 30 uL LowTE | ||
*Qubit: ''' | *Qubit: '''3.1''' ng/μL | ||
*Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2017-06-22) | *Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2017-06-22) | ||
[[ | [[Image:ZOLA P4 Bioanalyzer.JPG|500px]] | ||
Latest revision as of 02:19, 27 September 2017
Project name | Main project page Previous entry Next entry |
BestRAD Library Prep - Silvereye Plate 4
1) 2uL 100bp low scale ladder, 3) 2uL Silvereye sheared DNA
1) 2uL 100bp low scale ladder 2) 5uL Silvereye PCR product
1) 2uL 100bp low scale ladder 2) 5uL Silvereye PCR product
|