CTR:Notebook/Migrant/2017/07/24: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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**BioRuptor NGS: 10 cycles of 15 seconds on, 90 seconds off, High Power | **BioRuptor NGS: 10 cycles of 15 seconds on, 90 seconds off, High Power | ||
**Combined both tubes of sheared DNA and ran on a gel | **Combined both tubes of sheared DNA and ran on a gel | ||
[[ | [[Image:NOFU P1 sheared 07252017.JPG|50px]] | ||
1) 2uL 100bp low scale ladder, | 1) 2uL 100bp low scale ladder, | ||
3) 2uL NOFU sheared DNA | 3) 2uL NOFU sheared DNA | ||
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****65°C for 75 seconds | ****65°C for 75 seconds | ||
***65°C for 5 minutes | ***65°C for 5 minutes | ||
[[ | [[Image:NOFU P1 testPCR 07262017.JPG|60px]] | ||
1) 2uL 100bp low scale ladder | 1) 2uL 100bp low scale ladder | ||
2) 5uL NOFU PCR product | 2) 5uL NOFU PCR product | ||
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****65°C for 75 seconds | ****65°C for 75 seconds | ||
***65°C for 5 minutes | ***65°C for 5 minutes | ||
[[ | [[Image:NOFU P1 finalPCR 07272017.JPG|60px]] | ||
1) 2uL 100bp low scale ladder | 1) 2uL 100bp low scale ladder | ||
2) 5uL NOFU PCR product | 2) 5uL NOFU PCR product | ||
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***Elute in 30 uL LowTE | ***Elute in 30 uL LowTE | ||
*Qubit: ''' | *Qubit: '''6.5''' ng/μL | ||
*Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2017-07-25) | *Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2017-07-25) | ||
[[ | [[Image:NOFU P1 BioanalyzerTrace.JPG|500px]] |
Latest revision as of 02:19, 27 September 2017
Project name | Main project page Previous entry Next entry |
BestRAD Library Prep - Northern Fulmar Plate 1
1) 2uL 100bp low scale ladder, 3) 2uL NOFU sheared DNA
1) 2uL 100bp low scale ladder 2) 5uL NOFU PCR product
1) 2uL 100bp low scale ladder 2) 5uL NOFU PCR product
|