CTR:Notebook/PIRE/2015/02/02: Difference between revisions
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Sirena Lao (talk | contribs) |
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****72 degrees for 30 seconds | ****72 degrees for 30 seconds | ||
***72 degrees for 5 minutes | ***72 degrees for 5 minutes | ||
[[Image:Birds3 pcr1 2015-02-03.JPG|200px]] | [[Image:Birds3 pcr1 2015-02-03.JPG|200px]]<br> | ||
1) 1μL Greenbul DNA template, 2) 5μL Greenbul PCR product, 3) 2 uL 100 bp low scale ladder, 4) 1μL Sunbird DNA template, 5) 5μL Sunbird PCR product | 1) 1μL Greenbul DNA template, 2) 5μL Greenbul PCR product, 3) 2 uL 100 bp low scale ladder, 4) 1μL Sunbird DNA template, 5) 5μL Sunbird PCR product | ||
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→Greenbul PCR does not look brighter than template, but may not be half as bright. | →Greenbul PCR does not look brighter than template, but may not be half as bright. Also, gel ran twice because first one failed, leaving only 40μL of PCR product left. | ||
2015-02-03: | 2015-02-03: |
Revision as of 11:48, 3 February 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - Greenbuls and Sunbirds Plate 3
2015-01-31:
2015-02-01:
1) 2μL sheared Greenbul DNA, 2) 2uL 100bp low scale ladder, 3) 2uL sheared Sunbird DNA 2015-02-02:
2015-02-03:
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