CTR:Notebook/PIRE/2015/04/01: Difference between revisions
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* Sonication (2015-04-30) | * Sonication (2015-04-30) | ||
**BioRuptor NGS: 8 cycles of 15 seconds on, 90 seconds off, High Power | **BioRuptor NGS: 8 cycles of 15 seconds on, 90 seconds off, High Power | ||
[[Image:Skinks2 sheared1 2015-04-30.jpg|150px]] | |||
<br> | |||
1) 2uL sheared DNA, | |||
2) 2uL 100bp low scale ladder | |||
*Blunt End Repair (2015-04-30) | *Blunt End Repair (2015-04-30) | ||
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*PCR amplification (2015-04-30) | *PCR amplification (2015-04-30) | ||
**NEBPCR on JOHN Block A | **NEBPCR on JOHN Block A | ||
[[Image:Skinks2 pcr 2015-04-30.JPG|150px]] | |||
<br> | |||
1) 2uL 100bp low scale ladder, | |||
2) 5uL of PCR product, | |||
3) 1uL of template | |||
*Qubit: 8.74 ng/uL | |||
*2nd PCR amplification (2015-05-01) | |||
**Used 10 uL of template | |||
**NEBPCR on JOHN Block A | |||
[[Image:Skinks2 pcr2 2015-05-01.jpg|150px]] | |||
<br> | |||
1) 2uL 100bp low scale ladder, | |||
2) 5uL of PCR product, | |||
*Picogreen: 5.43 ng/uL | |||
*Qubit: 9.82 ng/uL | |||
Revision as of 15:34, 1 May 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - Skinks Plate 2
1) 2uL 100bp low scale ladder,
2) 2uL sheared DNA
1) 1 uL DNA template, 2) 5 uL PCR product, 3) 2 μL 100bp ladder
1) 5 uL PCR product, 2) 1 μL template, 3) 2 μL 100bp ladder
RAD Library Re-prep - Skinks Plate 2Reprep from plate.
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