CTR:Notebook/PIRE/2015/08/25: Difference between revisions
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[[Image:PCRproduct08272015 2.JPG|200px]] | [[Image:PCRproduct08272015 2.JPG|200px]] | ||
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product<br> | 1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product<br> | ||
→Successful amplification. Clean up, | →Successful amplification. | ||
*Clean up (2015-09-02, with Mice Plate 1) | |||
**45μL beads | |||
**Added a second cleanup using ~28μL beads | |||
**Ran gel to see final product | |||
[[Image:Libraries-skinksP3 miceP1.jpg|200px]] | |||
2μL Skink library on left | |||
*Picogreen: 1.34 ng/μL | |||
*BioAnalyzer | |||
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Revision as of 09:37, 3 September 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - Skinks Plate 3 (48 wells, Reruns)
1) 2uL 100bp low scale ladder,
2) 2uL sheared DNA
1) 2uL 100bp low scale ladder, 2) 2uL sheared DNA
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product →Visible amplification, but size range difficult to discern.
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product
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