CTR:Notebook/PIRE/2015/08/31: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PIRE RAD Library Preps</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> PIRE RAD Library Preps</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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| colspan="2"| | | colspan="2"| | ||
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* Sonication (2015-09-01) | * Sonication (2015-09-01) | ||
**BioRuptor NGS: 12 cycles of 30 seconds on, 90 seconds off, High Power | **BioRuptor NGS: 12 cycles of 30 seconds on, 90 seconds off, High Power | ||
[[Image:Sheared_09012015.jpg| | [[Image:Sheared_09012015.jpg|350px]] <br> | ||
1) 2μL sheared Butterfly DNA<br> | 1) 2μL sheared Butterfly DNA<br> | ||
3) 2μL sheared Frog DNA<br> | 3) 2μL sheared Frog DNA<br> | ||
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****72 degrees for 30 seconds | ****72 degrees for 30 seconds | ||
***72 degrees for 5 minutes | ***72 degrees for 5 minutes | ||
[[Image:PCR 09012015.jpg| | [[Image:PCR 09012015.jpg|400px]]<br> | ||
# 1μL Butterfly template | # 1μL Butterfly template | ||
# 5μL Butterfly PCR product | # 5μL Butterfly PCR product | ||
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*** 1 uL P2 adapter primer (25 uM) | *** 1 uL P2 adapter primer (25 uM) | ||
[[Image:PCR2 mice 09022015.jpg|200px]] | [[Image:PCR2 mice 09022015.jpg|200px]] | ||
1) 2μL 100bp low scale ladder, 2) 1μL Mouse template, 3) 5μL Mouse PCR product | 1) 2μL 100bp low scale ladder, 2) 1μL Mouse template, 3) 5μL Mouse PCR product<br> | ||
→ Clear amplification. | → Clear amplification. | ||
Latest revision as of 01:06, 27 September 2017
PIRE RAD Library Preps | Main project page Previous entry Next entry |
RAD Library Prep - 48-sample libraries for Butterflies, Frogs, Mice, Skinks
→Amplification failed for Butterflies, Frogs, Skinks. Mouse shows some amplification.
1) 2μL 100bp low scale ladder, 2) 1μL Mouse template, 3) 5μL Mouse PCR product
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