CTR:Notebook/PIRE/2015/08/31: Difference between revisions
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* Sonication (2015-09-01) | * Sonication (2015-09-01) | ||
**BioRuptor NGS: 12 cycles of 30 seconds on, 90 seconds off, High Power | **BioRuptor NGS: 12 cycles of 30 seconds on, 90 seconds off, High Power | ||
[[Image:Sheared_09012015.jpg| | [[Image:Sheared_09012015.jpg|350px]] <br> | ||
1) 2μL sheared Butterfly DNA<br> | 1) 2μL sheared Butterfly DNA<br> | ||
3) 2μL sheared Frog DNA<br> | 3) 2μL sheared Frog DNA<br> | ||
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****72 degrees for 30 seconds | ****72 degrees for 30 seconds | ||
***72 degrees for 5 minutes | ***72 degrees for 5 minutes | ||
[[Image:PCR 09012015.jpg| | [[Image:PCR 09012015.jpg|400px]]<br> | ||
# 1μL Butterfly template | # 1μL Butterfly template | ||
# 5μL Butterfly PCR product | # 5μL Butterfly PCR product | ||
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*** 1 uL P2 adapter primer (25 uM) | *** 1 uL P2 adapter primer (25 uM) | ||
[[Image:PCR2 mice 09022015.jpg|200px]] | [[Image:PCR2 mice 09022015.jpg|200px]] | ||
1) 2μL 100bp low scale ladder, 2) 1μL Mouse template, 3) 5μL Mouse PCR product | 1) 2μL 100bp low scale ladder, 2) 1μL Mouse template, 3) 5μL Mouse PCR product<br> | ||
→ Clear amplification. | → Clear amplification. | ||
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2μL Mouse library on right | 2μL Mouse library on right | ||
<br> | <br> | ||
*PicoGreen: 1. | *PicoGreen: 1.54 ng/μL | ||
<br> | <br> | ||
*Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2015-09-03) | *Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2015-09-03) | ||
[[Image:BioAnalyzer MiceP1 2015-09-03.jpg|550px]] | |||
*Sent 10nM to Berkeley for sequencing (Illumina HiSeq 100 PE) (2015-09-03) | *Sent 10nM to Berkeley for sequencing (Illumina HiSeq 100 PE) (2015-09-03) |
Revision as of 10:16, 3 September 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - 48-sample libraries for Butterflies, Frogs, Mice, Skinks
→Amplification failed for Butterflies, Frogs, Skinks. Mouse shows some amplification.
1) 2μL 100bp low scale ladder, 2) 1μL Mouse template, 3) 5μL Mouse PCR product
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