CTR:Notebook/PIRE/2015/08/31: Difference between revisions
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→ Clear amplification. | → Clear amplification. | ||
*Bead | *Bead clean up (2015-09-02, with Skinks plate 3) | ||
**45μL beads | **Use 45μL AMPure beads (1:1) | ||
**Added second clean up using ~28μL beads (1:1) | |||
[[Image:Libraries-skinksP3 miceP1.jpg|200px]] | **Ran gel for final product | ||
[[Image:Libraries-skinksP3 miceP1.jpg|200px]] | |||
2μL Mouse library on right | |||
<br> | <br> | ||
*PicoGreen: 1.64 ng/μL | *PicoGreen: 1.64 ng/μL | ||
<br> | |||
*Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2015-09-03) | *Run 3μL on BioAnalyzer DNA High Sensitivity Chip (2015-09-03) | ||
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* | *Sent 10nM to Berkeley for sequencing (Illumina HiSeq 100 PE) (2015-09-03) | ||
Revision as of 09:55, 3 September 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
RAD Library Prep - 48-sample libraries for Butterflies, Frogs, Mice, Skinks
1) 2μL sheared Butterfly DNA, 3) 2μL sheared Frog DNA, 5) 2μL sheared Mouse DNA, 7) 2μL sheared Skink DNA
→Amplification failed for Butterflies, Frogs, Skinks. Mouse shows some amplification.
1) 2μL 100bp low scale ladder, 2) 1μL Mouse template, 3) 5μL Mouse PCR product → Clear amplification.
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