CTR:Notebook/PIRE/Entry Base: Difference between revisions
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Sirena Lao (talk | contribs) |
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→Successful amplification. Clean up, quant, and Bioanalyzer will be done with other libraries. | →Successful amplification. Clean up, quant, and Bioanalyzer will be done with other libraries. | ||
*Bead clean up | |||
* | **Use 45 uL AMPure beads (1:1) | ||
**2 washes of 200 uL 80% EtOH | |||
**Elute in 30 uL LowTE | |||
<br> | |||
*PicoGreen: 3.15 ng/μL | *PicoGreen: 3.15 ng/μL | ||
Revision as of 09:19, 31 August 2015
PIRE RAD Library Preps | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page |
RAD Library Prep - Skinks Plate 3 (48 wells, Reruns)
1) 2uL 100bp low scale ladder,
2) 2uL sheared DNA
1) 2uL 100bp low scale ladder, 2) 2uL sheared DNA
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product →Visible amplification, but size range difficult to discern.
1) 2 uL 100 bp low scale ladder, 2) 1 uL DNA template, 3) 5 uL PCR product
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