Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-10
James Field: Aims:
Morning: 1) Finish extracting BioBricks from Registry.
- BBa_R0011
- BBa_K118011
- BBa_I0500
2) Train up a Bioengineer and begin promoter testing.
Results:
AM:
Made a start on colanic acid wet lab prep. Deadline for completion set to Wednesday.
PM:
Extracted BioBricks from registry as planned.
--David Roche 12:14, 10 August 2009 (EDT)
Achievements
Focused on trehalose section of Module 2:
Discussed with James and Nuri in the morning the design rationale of Module 2.
Came up with a list of criteria to be filled in for each section - trehalose. Worked on developing trehalose assay
Royah Vaezi 17:28, 9 August 2009 (EDT):
Aims:
1)Finish extracting BioBricks from Registry with James/show bioengineers.
- BBa_R0011
- BBa_K118011
- BBa_I0500
2)Train up a bioengineer and begin PCR for cloning & primer design for SLIC.
- Charles Fracchia 05:08, 10 August 2009 (EDT):
Aims:
1) List all jobs and assign them
2) Decide on website architecture and implement foundations
- Decide on technologies used:
- Flash
- Javascript
- Create browser-selective script
- Create Flash to Javascript messages/switch
- Charles Fracchia 05:08, 10 August 2009 (EDT):
Aims:
1) MATLAB tutorial
- 1h or 2
2) Timer design: DRYLAB only?
- James: Implement Glucose/Arabinose in the lab as well as model it
- Kun: No wetlab implementation
- Dave:
- Nuri: not clear, not spend too much
- TianYi: timer is not essential, focus more on encapsulation
- Dineka: give priority encapsulation
- Characterise the outputs of experiments
- Aim for step by step approach
- Journal Club: Nuri send paper round
- Summarise the set of data that we are getting from experiments, with testing constructs and everything
- Nuri Purswani 09:49, 10 August 2009 (EDT):
Aims
- Help out with planning constructs for M2 (relationship between [glucose] and CRP.
- Modelling considerations: Dry lab plan
Weekly aims:
- Organize wiki
- Send list of people and availability through september Done but needs discussion
Updated weekly aims
Split up into modules and fill in the following criteria: DNA, assembly, assays, output data from experiments, shopping list for each protocol, modelling perspectives. Module navigation
Module 1
DNA constructs Done
Assembly Needs to be revised for SLIC usage
Assays Done, Protocols transcribed to OWW pages
Output Data Give feedback plz
Shopping list In progress
Modelling perspectives Needs detailing
Module 2
Split up :
Nuri - Promoter Characterisation
James - Colanic Acid
Dave - Trehalose Production
DNA constructs
JF Done Please Feedback
DR Done Please Feedback
Assembly
DR : Not yet done,
Not Done - JF
Assays
DR : In Progress,
JF in progress - Please Feedback
Output Data
DR : In Progress (feedback)
JF in progress - Please Feedback
Shopping list
DR : Done (feedback)
JF Done Please Feedback
Modelling perspectives
DR : done
In Progress, JF in progress - Please Feedback
Module 3
Split up :
Promoter
Thermoinduction - Kun
Killing - Dineka
Methylation
DNA constructs
KX Done Please Feedback
DK Done Please Feedback
Assembly
KX & DK: Not yet done.
Assays
KX : Done - Beta gal & temperature shift.
DK : Done - Staining. CFU in progress.
Output Data
KX & DK : In Progress
Shopping list
KX : Done - Beta gal & temperature shift.
DK : Done - Staining. CFU in progress.
Modelling perspectives
KX & DK : In progress.