Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-17: Difference between revisions
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Royah Vaezi (talk | contribs) No edit summary |
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PRIMERS: Royah, James | PRIMERS: Royah, James | ||
<b>[[User:Royah Vaezi|Royah Vaezi]]< | <b>[[User:Royah Vaezi|Royah Vaezi]]</b> | ||
*Gel extraction of AK3 vector and B3023. | *Gel extraction of AK3 vector and B3023. | ||
*Dephosphorylation of vector and ON ligation to B3023 at 4°C. | *Dephosphorylation of vector and ON ligation to B3023 at 4°C. | ||
*PCR of RcsB from Top 10 cells. Failed so will try again with different aliquot of Top 10s. | *PCR of RcsB from Top 10 cells. Failed so will try again with different aliquot of Top 10s. | ||
*ON restriction digests of OtsA, OtsB and Dam. | *ON restriction digests of OtsA, OtsB and Dam. |
Latest revision as of 03:00, 21 August 2009
- Nuri Purswani 14:11, 16 August 2009 (EDT):
- Modelling directions for M0 and M1
- Browsing through previous igem modelling pages
- Meeting with Matthieu 2pm to discuss drylab plan
M0:
Assays: Dineka, Kun
Modelling: Nuri
Cloning: James
M1
Assays: Charles
Modelling: Nuri
Cloning: Charles
M2
Assays: Dave (Trehalose), James (Colanic acid)
Modelling: Tianyi
Cloning: Dave (Trehalose), James (Colanic acid)
M3
Assays: Dineka, Kun
Modelling: Dineka, Kun
Cloning: Dineka, Kun
PRIMERS: Royah, James
- Gel extraction of AK3 vector and B3023.
- Dephosphorylation of vector and ON ligation to B3023 at 4°C.
- PCR of RcsB from Top 10 cells. Failed so will try again with different aliquot of Top 10s.
- ON restriction digests of OtsA, OtsB and Dam.