Calendar:IGEM:IMPERIAL/2009/Calendar:TeamProgress/2009-8-21: Difference between revisions
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*Tianyi and Nuri: -Research enzyme kinetics for cellulase and PAH. | *Tianyi and Nuri: -Research enzyme kinetics for cellulase and PAH. | ||
- Effect of secondary carbon sources in MO. | - Effect of secondary carbon sources in MO. | ||
<b>[[User:Royah Vaezi|Royah Vaezi]]</b> | |||
AIMS: | |||
*Gel extract and purify RcsB, OtsA and Dam. | |||
*Run extracts on gel with vector to determine ratios. | |||
*Ligate inserts to vector (AK3) and leave over the weekend at 4°C. | |||
Revision as of 03:14, 21 August 2009
- Nuri Purswani 04:57, 21 August 2009 (EDT): Goals for the Drylab
- MO (linked also to M2): Investigate the effect of the secondary carbon source on bacteria.
- M1:
- Enzyme literature review.
- IPTG toxicity: Include effects on model and go to wetlab to get OD data
- M2: Research on modelling we can do. Also related to MO modelling.
- M3: Review killing model. See if we can find some real data.
Goals for today-
- Charles and Kun: Implement M3 killing model.
- Tianyi and Nuri: -Research enzyme kinetics for cellulase and PAH.
- Effect of secondary carbon sources in MO.
Royah Vaezi AIMS:
- Gel extract and purify RcsB, OtsA and Dam.
- Run extracts on gel with vector to determine ratios.
- Ligate inserts to vector (AK3) and leave over the weekend at 4°C.
