Calendar:ImperialWetLabNotebook2009iGEM/2009-9-3: Difference between revisions
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Royah Vaezi (talk | contribs) (New page: ===Tasks for today=== *Midi-preps on cellulases and RFP (<i>DK & JF</i>) *Run WaaL on gel, gel extract, purify and run against XbaI/SpeI AK3 vector (<i>Jim</i>) *Ligations - Harvard BB, C...) |
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*Midi-preps on cellulases and RFP (<i>DK & JF</i>) | *Midi-preps on cellulases and RFP (<i>DK & JF</i>) | ||
*Run WaaL on gel, gel extract, purify and run against XbaI/SpeI AK3 vector (<i>Jim</i>) | *Run WaaL on gel, gel extract, purify and run against XbaI/SpeI AK3 vector (<i>Jim</i>) | ||
*Ligations - Harvard BB, CRP, dirty ligation (RBS & AK3) (<i>DK</>) | *Ligations - Harvard BB, CRP, dirty ligation (RBS & AK3) (<i>DK</i>) | ||
*Mini-prep and RD on OtsB (<i>RV</i>) | *Mini-prep and RD on OtsB (<i>RV</i>) | ||
*Replenish DNA insert stocks - OtsA, OtsB, Dam, RcsB (<i>RV</i>) | *Replenish DNA insert stocks - OtsA, OtsB, Dam, RcsB (<i>RV</i>) | ||
Revision as of 07:59, 3 September 2009
Tasks for today
- Midi-preps on cellulases and RFP (DK & JF)
- Run WaaL on gel, gel extract, purify and run against XbaI/SpeI AK3 vector (Jim)
- Ligations - Harvard BB, CRP, dirty ligation (RBS & AK3) (DK)
- Mini-prep and RD on OtsB (RV)
- Replenish DNA insert stocks - OtsA, OtsB, Dam, RcsB (RV)
- Re-transform all BBs (JF)
- Digest CRP, HVD, GFP, run on gel and gel purify GFP (DK)
- Gel purify CRP insert (Jim)
- Make more LB & LB Agar (RV)
- Make Kan & Amp plates (DR)
