Calendar:TeamProgress/2009-8-13

--David Roche 10:55, 13 August 2009 (EDT)
PCR monkey

• James Field 13:32, 13 August 2009 (EDT):

• Colanic acid assay 2.1 protocol - completed
• Completed miniprep and confirmed that BioBricks taken out of registry were correct.

• Charles Fracchia 13:41, 13 August 2009 (EDT):
• Explained thoroughly assays and genetic circuits
• Started modelling on LacI repressible promoter
• Designed site directed mutagenesis primers for PAH serine 16 -> Glutamine
• Sacrificed a sheep for good luck
• Beefed up the description of PAH activity assay and added step 0 of sonication to the protocol
• Helped loading Gel electrophoresis for biobrick checking

• Kun Xue 15:25, 13 August 2009 (EDT):

• Researched on cI protein sequences and how the Harvard cI sequence might be wrong
• Beefed up description of Cellulase assay and output data

Royah Vaezi:

• Midi-prep for PAH. Last steps to be completed tomorrow.
• PCR of OtsA and Dam. Re-did OtsB PCR using mid-temp PCR product from James C's last round of PCR. Will run all PCR products gained on a gel tomorrow AM to check.
• Restriction digest of B3023 with XbaI and SpeI (overnight). Vector will be digested tomorrow ready for ligation. Aim is to have the BioBrick on Tues...