Cameron M. Rehmani Seraji Week 10: Difference between revisions
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*'''What strain(s) of yeast did they use? Was the strain haploid or diploid?''' | *'''What strain(s) of yeast did they use? Was the strain haploid or diploid?''' | ||
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Revision as of 20:17, 28 March 2017
Electronic Lab Notebook Week 10
Purpose
Part 1
Biological Terms
- Trehalose:A nonreducing disaccharide, also known as mushroom sugar or mycose, that acts as a reserve carbohydrate in certain fungi (especially yeasts), algae, and lichens.
- Mannoproteins:Yeast cell wall components that are proteins with large numbers of mannose groups attached; highly antigenic.
- Desaturase:Any of several enzymes that putdouble bonds into the hydrocarbon areas of fatty acids.
- Transcriptome:the entire set of mRNAs transcribed from a cell's genome.
- In vivo:Occurring or made to occur within a living organism; literally it means ‘in life’.
- Orthologues:a gene, protein, or biopolymeric sequence that is evolutionarily related to another by descent from a common ancestor, having diverged as a result of a speciation event.
- Exogenous:Developed or originating outside the organism, as exogenous disease.
- Motif:The smallest group of atoms in a polymer that, when under the influence of a rotation-translation operator, will assemble the rest of the atoms in the chain.
- Diurnal:Refers to the condition of being active during the day as observed in the behavior of certain plants and animals.
- Prototrophic Strains:Strain's that have the same nutritional requirements as the wild-type strain.
Part 2
Outline
- What is the main result presented in this paper?
- The overall main result presented in this paper is that S. cerevisiae will have different transcriptional responses to low temperature and low specific growth rate when it is grown in batch cultures and in chemostat cultures.
- The use of a chemostat cultures helps isolate and study the different physiological phases of S. cerevisiae and its ability to adapt to environmental stress.
- What is the importance or significance of this work?
- The importance of this work is
- Create a flow chart to describe their methods. Answer the following questions if they are relevant to your article.
- Grow S. cerevisiaein a chemostat --> use of cold shock (12°C) or use of heat shock (30°C) --> limit glucose or limit ammonia
- This results in there being four types of growth conditions:
- 12°C, glucose-limited
- 12°C, ammonium-limited
- 30°C, glucose-limited
- 30°C, ammonium-limited
- How did they treat the cells (what experiment were they doing?)
- The goal of the study was to investigate steady-state growth of S. cerevisiae at suboptimal temperatures, with emphasis on genome-wide transcriptional regulation.
- What strain(s) of yeast did they use? Was the strain haploid or diploid?
- What media did they grow them in? Under what conditions and temperatures?
- What controls did they use?
- How many replicates did they perform per condition?
- What mathematical/statistical method did they use to analyze the data?
- What transcription factors did they talk about?
- Table 1
- Figure 1
- Figure 2
- Table 2
- Table 3
- Figure 3
- Figure 4
- Figure 5
- Figure 6
Acknowledgments
- Except for what is noted above, this individual journal entry was completed by me and not copied from another source.
References
- Week 10 Assignment Page
- Tai, S. L., Daran-Lapujade, P., Walsh, M. C., Pronk, J. T., & Daran, J. M. (2007). Acclimation of Saccharomyces cerevisiae to low temperature: a chemostat-based transcriptome analysis. Molecular biology of the cell, 18(12), 5100-5112. doi: 10.1091/mbc.E07-02-0131
- Cameron M. Rehmani Seraji
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