Cameron M. Rehmani Seraji Week 6

Electronic Lab Notebook Week 6

Cameron M. Rehmani Seraji

Purpose

• The purpose of the week 6 assignment is to construct models of the dynamics of yeast, nitrogen, and carbon. This will be accomplished by further studying the growth of yeast and how the state variables and parameters affect it. In particular, this assignment will focus on the central nitrogen metabolism processes project.

Methods

• For this assignment, I decided to focus on the central nitrogen metabolism processes project.
• From the three readings, I selected the ter Schure et al 1995 paper and took notes.
  • ter Schure et al 1995 Notes

Results

Hypothesis

• An increase in the ammonia feed rate would cause the yield of S. cerevisiae to remain constant. S. cerevisiae, ammonia, and glucose fit into the multiplicative nutrient use model because ammonia and glucose are both needed for S. cerevisiae to grow. The yield is constant because the residual ammonia concentration becomes limiting at dilution rates greater than 0.15 h^-1.

State Variables

• y=concentration of yeast
• c=concentration of nutrient (ammonia or glucose)

Explanation of Differential Equations

• Ammonia
• Glucose
• Yeast

Acknowledgements

• Worked with Lauren M. Kelly in Seaver 120 on February 22nd.
• Except for what is noted above, this individual journal entry was completed by me and not copied from another source.

References

• Dahlquist, Kam D. (2017) BIOL398-05/S17:Week 6. Retrieved from http://www.openwetware.org/wiki/BIOL398-05/S17:Week_6 on 22 February 2017.
• Schure, E. G., Sillje, H. H., Raeven, L. J., Boonstra, J., Verkleij, A. J., & Verrips, C. T. (1995). Nitrogen-regulated transcription and enzyme activities in continuous cultures of Saccharomyces cerevisiae. Microbiology, 141(8), 2019-2019. doi:10.1099/13500872-141-8-2019

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