Carboxyfluorescein Diacetate

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  • fluorescein is formed by intracellular hydrolysis of CFDA
  • carboxyfluorescein contains extra negative charges (structure) and is therefore better retained in cells
  • been used in bacteria
  • CFDA is moderately permeant to most cell membranes with uptake greater at pH 6.2 than at pH 7.4
  • Oregon Green 488 (496/524) carboxylic acid diacetate is a more photostable version and less pH sensitive
  • timing issues with how long you have to wait for the compound to be hydrolyzed ?
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