Carrico:Recipes

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===2XYT Media (to make 1L)===
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==='''2XYT Media (to make 1L)'''===
#Combine: 900mL distilled water, 16g Bacto Tryptone, 10g Bacto Yeast Extract, 5g NaCl
#Combine: 900mL distilled water, 16g Bacto Tryptone, 10g Bacto Yeast Extract, 5g NaCl
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#Autoclave
#Autoclave
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==DNA Gel Electrophoresis==
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=='''DNA Gel Electrophoresis'''==
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===50x TAE Buffer (to make 1L)===
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==='''50x TAE Buffer (to make 1L)'''===
*242g 2M Tris base
*242g 2M Tris base
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*Add water up to 1L
*Add water up to 1L
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Note: To dilute 50x to 1x, add 20mL of 50x to 980mL water
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'''Note:''' To dilute 50x to 1x, add 20mL of 50x to 980mL water

Revision as of 23:40, 14 July 2009


Carrico Lab at Stony Brook


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2XYT Media (to make 1L)

  1. Combine: 900mL distilled water, 16g Bacto Tryptone, 10g Bacto Yeast Extract, 5g NaCl
  2. pH to 7.0
  3. Add water up to 1L
  4. Autoclave

DNA Gel Electrophoresis

50x TAE Buffer (to make 1L)

  • 242g 2M Tris base
  • 57.1mL 1M glacial acetic acid
  • 200mL 100mM EDTA
  • Add water up to 1L

Note: To dilute 50x to 1x, add 20mL of 50x to 980mL water

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