Carrico:Recipes
From OpenWetWare
Carrico Lab at Stony Brook
Home Lab Members Recipes Standard Protocols Publications Links
2XYT Media (to make 1L)
- Combine: 900mL distilled water, 16g Bacto Tryptone, 10g Bacto Yeast Extract, 5g NaCl
- pH to 7.0
- Add water up to 1L
- Autoclave
DNA Gel Electrophoresis
50x TAE Buffer (to make 1L)
- 242g 2M Tris base
- 57.1mL 1M glacial acetic acid
- 200mL 100mM EDTA
- Add water up to 1L
Note: To dilute 50x to 1x, add 20mL of 50x to 980mL water