Carrico:Standard Protocols: Difference between revisions
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#Paste-in the sequence | #Paste-in the sequence | ||
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#Double click on newly created plasmid | #Double-click on newly created plasmid | ||
===How to create a gene in a plasmid=== | ===How to create a gene in a plasmid=== | ||
Revision as of 14:17, 22 September 2008
Carrico Lab at Stony Brook
Home Lab Members Recipes Standard Protocols Publications Links
QIAquick Gel Extraction Kit Protocol (using a microcentrifuge) - pdf file
"How to" for Vector NTI
How to create a new plasmid
- Open the Vector NTI Explorer program
- Click on the "New" icon
- Fill-in "Name"
- Under the "Sequence" tab, click on "Edit Sequence"
- Paste-in the sequence
- Select "OK"
- Double-click on newly created plasmid
How to create a gene in a plasmid
- Highlight the plasmid you would like edited
- Select "Edit" --> "New" --> "Add feature"
- Select the name of the plasmid
- Select "Save as"
How to change nucleotides
- Highlight bases to be changed
- Select "Edit" --> "New" --> "Replace"
- "Save As" a different name
