Cconboy:Promoter Characterization: Difference between revisions

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===Background===
===Background===


The rational design of integrated biological systems requires a set of characterized, compatible components. Here we attempt to improve the characterization of a set of transcriptional promoters contained in the registry of standard biological parts. We measure the relative strength of sixteen promoters by flow cytometry, via expression of a YFP reporter, measured by flow cytometry.
The rational design of integrated biological systems requires a set of characterized, compatible components. Here we attempt to improve the characterization of a set of transcriptional promoters contained in the registry of standard biological parts. We measure the relative strength of sixteen promoters via expression of a YFP reporter, measured by flow cytometry and plate reader.


*[[cconboy:Promoter Characterization/Constructs|Constructs]]
*[[cconboy:Promoter Characterization/Constructs|Constructs]]

Revision as of 12:05, 22 March 2006

Caitlin Conboy, Jen Braff, Drew Endy

Experiments and analysis performed 04.04 - 07.04

Background

The rational design of integrated biological systems requires a set of characterized, compatible components. Here we attempt to improve the characterization of a set of transcriptional promoters contained in the registry of standard biological parts. We measure the relative strength of sixteen promoters via expression of a YFP reporter, measured by flow cytometry and plate reader.

Related

PoPS device screening plasmid

  • Subsequent part characterization work by Jason Kelly, Josh Michener and Kelly Chang