Cconboy:Terminator Characterization: Difference between revisions
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Experiments performed by Caitlin Conboy, Fall 2003 | Experiments performed by Caitlin Conboy, Fall 2003 | ||
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[[cconboy:Terminator Characterization/Methods|Protocol]] | [[cconboy:Terminator Characterization/Methods|Protocol]] | ||
[[cconboy:Terminator Characterization/Results|Results]] | [[cconboy:Terminator Characterization/Results|Results and Discussion]] | ||
===Related=== | ===Related=== | ||
Revision as of 12:13, 11 April 2006
Experiments performed by Caitlin Conboy, Fall 2003
Background
The rational design of integrated biological systems requires a set of characterized, compatible components. Here we characterize a set of transcriptional terminators contained in the registry of standard biological parts. [1]. We measure termination efficiency by inserting each terminator between the coding regions for CFP and YFP on a polycistronic message and measuring the fluorescence ratio of the two fluorophores by flow cytometry.
Related
- Subsequent terminator characterization work by Jason Kelly, Josh Michener and Kelly Chang.
