Cell and tissue lysis hub

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[[Category:Escherichia coli]][[Category:Mouse]][[Category:Streptomyces]][[Category:Yeast]]

Revision as of 06:30, 26 June 2008

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.


Comparison of lysis methods


  • most efficient method of cell fractionation
  • problem: heat build up which can denature proteins (proportionate to length of sonication)
  • precaution: do on ice and sonicate intermittantly


  • best for animal tissue; less suitable for cells
  • precaution: do on ice to reduce heat build-up and denaturation


  • least effective method
  • plus: does not denature proteins as much as other methods


  • chemical method of lysis
  • problems: detergent may inhibit subsequent reactions
  • problems: detergent may disrupts protein interactions

Specific protocols

Related discussions on BioForum

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