Cell and tissue lysis hub

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(categories)
(categories)
Line 35: Line 35:
-
[[Category:Protocol]][[Category:Material]]
+
[[Category:Protocol]][[Category:DNA]][[Category:RNA]][[Category:protein]]
-
[[Category:Escherichia]]
+
[[Category:Material]][[Category:Chemical]]
 +
[[Category:Escherichia coli]][[Category:Mouse]][[Category:Streptomyces]][[Category:Yeast]]

Revision as of 05:30, 26 June 2008

This page should point you to the many different general and lab-specific protocols describing tissue and cell lysis and serve as forum for comparison.

Contents

Comparison of lysis methods

Sonication

  • most efficient method of cell fractionation
  • problem: heat build up which can denature proteins (proportionate to length of sonication)
  • precaution: do on ice and sonicate intermittantly

Homogenisation

  • best for animal tissue; less suitable for cells
  • precaution: do on ice to reduce heat build-up and denaturation

Freeze-thaw

  • least effective method
  • plus: does not denature proteins as much as other methods

Detergents

  • chemical method of lysis
  • problems: detergent may inhibit subsequent reactions
  • problems: detergent may disrupts protein interactions

Specific protocols

Related discussions on BioForum

Personal tools