Cellular and molecular bioengineering laboratory

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'''(1) Fluorescence based biodetection & bioimaging'''
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Welcome to the Pam Silver lab wiki in [[Main_Page | OPENWETWARE]]. 
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== Research Information ==
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Both down-conversion and up-conversion fluorescent inorganic nanoparticles (quantum dots, lanthanide doped nanocrystals) are synthesized and used as fluorescent labels or imaging probes for biodetection & bioimaging. The up-conversion fluorescent nanoparticles can convert near infrared (NIR) light to visible light. Compared to conventional down-conversion fluorescent materials such as organic dyes and quantum dots, these nanoparticles have the following advantages: High light penetration depth in tissues; No photodamage to living organisms; Weak autofluorescence from cells or tissues; Low background light and high sensitivity for detection.
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* [[Silver: Synthetic Biology | Synthetic Biology]]
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* [[Silver: Genome Organization | Genome Organization]]
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* [[Silver: Pathways in Disease | Pathways  in Disease ]]
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* [[Silver: RNA Stuff | RNA Dynamics]]
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* [[Silver: Publications | Publications]]
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* [[Silver: In the News | In the News]]
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*[http://hibie.harvard.edu/ Harvard Institute of Biologically Inspired Engineering]
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*[http://www.msi.harvard.edu/ Microbial Sciences Institute]
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*[http://environment.harvard.edu/ Harvard Center for the Environment]
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*[http://www.bionumbers.org/ BioNumbers]
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Li ZQ & Zhang Y, Angewandte Chemie International Edition 2006, 45, 7732-7735.
 
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'''(2) Imaging-guided cancer therapy'''
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== Resources ==
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* [[Silver: Protocols | Protocols]]
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** [[Silver: Protocols#Genome Organization | Genome Organization]]
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** [[Silver: Protocols#RNA .22Stuff.22 | RNA]]
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** [[Silver: Protocols#Synthetic Biology | Synthetic Biology]]
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** [[Silver: Protocols#Cell-Based Screens | Cell-Based Screens ]]
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** [[Silver: Protocols#Methylation | Methylation]]
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** [[Silver: Protocols#Other .2F More General | Other / More General]]
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Multi-color fluorescent quantum dots and magnetic agents are encapsulated within nanometer-sized (~50 nm) chitosan nanoparticles. The small size of the nanoparticles allows them to be used as a labeling tag, at the same time, as a contrast agent in magnetic resonance imaging (MRI) as well. In the labeling of cancer cells, specific targeting molecules that recognize cancer cells can be attached to the surface of the nanoparticles so that they bind onto the surface of the cancer cells specifically. This can potentially help in the localization and identification of a cancerous tissue. Moreover, these nanoparticles can be used to deliver therapeutic drugs, proteins and genes by intravenous, oral and mucosal administration. Using these nanoparticles, drugs or genes can be precisely delivered to the specific cells or specific regions of tissues with aid of imaging techniques, for various applications.
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==Educational Programs==
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*[http://sysbiophd.med.harvard.edu/ Harvard University PhD Program in Systems Biology]
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*[http://www.hms.harvard.edu/dms/bbs/ Biological and Biomedical Sciences Graduate Program]
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*[http://arep.med.harvard.edu/biophysics/ Harvard University Biophysics Program]
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*[http://chembio.med.harvard.edu/ Harvard Chemical Biology Graduate Program]
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*[http://openwetware.org/wiki/IGEM:Harvard/2008 Harvard University iGEM team]
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*[http://bbf.openwetware.org/ The Biobricks Foundation]
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Tan WB & Zhang Y, Advanced Materials 2005, 17, 2375-2380.
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Tan WB, Jiang S & Zhang Y. Biomaterials, in press
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== Lab Information ==
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* [[Silver: Lab Members | Lab Members]]
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* [[Silver: Lab Meeting Shedule | Lab Meeting Schedule]]
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* [[Silver: Former Lab Members | Former Lab Members]]
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* [[Silver: Contact Information | Contact, Strain Requests and Joining the Group]]
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* [[Silver: Open Positions| Open Positions]]
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* [https://silver.med.harvard.edu/passwd/wiki/index.php?title=Main_Page Internal Lab Links (private)]
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**(private link: includes contact info, birthdays and pictures)
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'''(3) Bead based microarrays for multiplexing bioassays'''
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Compared to the microarrays fabricated on planar substrates, bead based microarrays are more robust as microbeads are ideal reagent delivery vehicles providing large reactive surface areas and have become omnipresent in biomedical applications. A technique is developed to fabricate a microfluidic device with unique dome-shape structures for high efficiency immobilization and patterning of single microbeads. We have also fabricated polymer porous films with tunable pore sizes by employing non-lithographic “breadth figure” method and colloidal template method, for patterning of microbeads. Our research aims to use arrays of encoded microbeads for high-throughput multiplexing bioassays.  
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Images on this page provided by Chris Brown and Caroline Ajo-Franklin. 
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Wiki Editing [[Silver: Wiki Instructions|Instructions]]
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Lu MH & Zhang Y, Advanced Materials 2006, 18, 3094-3098.
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Lab members, also visit the [https://silver.med.harvard.edu/passwd/wiki/index.php?title=Main_Page secure wiki].
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'''(4) Micropatterning of proteins & cells via self-assembled nanoparticles'''
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Micropatterning of biomolecules forms the basis of cell culture, biosensor and microarray technology. We have reported methods to pattern biomolecules through self-assembling polystyrene nanoparticles in arrayed microwells on a solid surface to form well-ordered patterning, followed by attaching biomolecules and cells to the assembled nanoparticles.  
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Wang C & Zhang Y, Advanced Materials 2005, 17, 150-153.
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Yap FL & Zhang Y, Langmuir 2005, 21, 5233-5236.
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Revision as of 12:36, 16 July 2008

Welcome to the Pam Silver lab wiki in OPENWETWARE.


Images on this page provided by Chris Brown and Caroline Ajo-Franklin.

Wiki Editing Instructions

Lab members, also visit the secure wiki.

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