Cellular and molecular bioengineering laboratory

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(New page: '''(1) Fluorescence based biodetection & bioimaging''' Both down-conversion and up-conversion fluorescent inorganic nanoparticles (quantum dots, lanthanide doped nanocrystals) are synth...)
Current revision (04:45, 3 September 2008) (view source)
 
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'''(1) Fluorescence based biodetection & bioimaging'''
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[[Cellular and Molecular Bioengineering Laboratory: Research| <font face="trebuchet ms" style="color:#000"> '''Research''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:People | <font face="trebuchet ms" style="color:#000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:Collobration | <font face="trebuchet ms" style="color:#000"> '''Collobration ''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:Publication | <font face="trebuchet ms" style="color:#000"> '''Publication''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:Opportunity | <font face="trebuchet ms" style="color:#000"> '''Opportunity ''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:Journal Club| <font face="trebuchet ms" style="color:#000"> '''Journal Club ''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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Both down-conversion and up-conversion fluorescent inorganic nanoparticles (quantum dots, lanthanide doped nanocrystals) are synthesized and used as fluorescent labels or imaging probes for biodetection & bioimaging. The up-conversion fluorescent nanoparticles can convert near infrared (NIR) light to visible light. Compared to conventional down-conversion fluorescent materials such as organic dyes and quantum dots, these nanoparticles have the following advantages: High light penetration depth in tissues; No photodamage to living organisms; Weak autofluorescence from cells or tissues; Low background light and high sensitivity for detection.
 
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== Multi-functional Nanocomposites for Biological Labeling and Diagnostics ==
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There have been tremendous advances in the development of in-situ labeling and screening of different biological entities, ranging from cells to DNAs. Many approaches have been developed for this purpose, such as chemical encoding with molecular tags, organic fluorophores, fluorescent colloids, and Raman fingerprints.
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Li ZQ & Zhang Y, Angewandte Chemie International Edition 2006, 45, 7732-7735.
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The development of labeling materials has been critically important. Some materials such as quantum nanodots, organic dyes and metal nanoparticles have been extensively used for biological labeling, however, they have to be surface modified to better suit their integration with biological systems. Recently, synthesis of monodisperse polymer nanospheres has stimulated great interest and incorporation of fluorophores in these nanospheres is particularly attractive.  
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In these nanocomposites, organic polymer can not only stabilize the nanoparticles in a solid matrix, but also effectively combine the peculiar features of organic and inorganic components and thus resulting in novel properties. These materials can bring new and unique capabilities to a variety of biomedical applications ranging from diagnosis of diseases to novel therapies.
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==Micropatterning of Biomolecules via Manipulation of Micro/Nano Spheres==
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'''(2) Imaging-guided cancer therapy'''
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Micropatterning of biomolecules, the attachment of biomoloecules within designated regions on solid surfaces while preventing nonspecific adhesion at other regions, forms the basis of microarray technnology. It has found many applications in various fields such as the diagnosis of disease, drug discovery, environmental testing, biological studies, etc.
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Multi-color fluorescent quantum dots and magnetic agents are encapsulated within nanometer-sized (~50 nm) chitosan nanoparticles. The small size of the nanoparticles allows them to be used as a labeling tag, at the same time, as a contrast agent in magnetic resonance imaging (MRI) as well. In the labeling of cancer cells, specific targeting molecules that recognize cancer cells can be attached to the surface of the nanoparticles so that they bind onto the surface of the cancer cells specifically. This can potentially help in the localization and identification of a cancerous tissue. Moreover, these nanoparticles can be used to deliver therapeutic drugs, proteins and genes by intravenous, oral and mucosal administration. Using these nanoparticles, drugs or genes can be precisely delivered to the specific cells or specific regions of tissues with aid of imaging techniques, for various applications.
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We are developing a new technique of micropatterning which allows the integration of non-planar spots on a planar microarray via anipulation of micro/nano spheres.
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Tan WB & Zhang Y, Advanced Materials 2005, 17, 2375-2380.
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Tan WB, Jiang S & Zhang Y. Biomaterials, in press
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'''(3) Bead based microarrays for multiplexing bioassays'''
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Compared to the microarrays fabricated on planar substrates, bead based microarrays are more robust as microbeads are ideal reagent delivery vehicles providing large reactive surface areas and have become omnipresent in biomedical applications. A technique is developed to fabricate a microfluidic device with unique dome-shape structures for high efficiency immobilization and patterning of single microbeads. We have also fabricated polymer porous films with tunable pore sizes by employing non-lithographic “breadth figure” method and colloidal template method, for patterning of microbeads. Our research aims to use arrays of encoded microbeads for high-throughput multiplexing bioassays.
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Lu MH & Zhang Y, Advanced Materials 2006, 18, 3094-3098.
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'''(4) Micropatterning of proteins & cells via self-assembled nanoparticles'''
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Micropatterning of biomolecules forms the basis of cell culture, biosensor and microarray technology. We have reported methods to pattern biomolecules through self-assembling polystyrene nanoparticles in arrayed microwells on a solid surface to form well-ordered patterning, followed by attaching biomolecules and cells to the assembled nanoparticles.
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Wang C & Zhang Y, Advanced Materials 2005, 17, 150-153.
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Yap FL & Zhang Y, Langmuir 2005, 21, 5233-5236.
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Current revision

Research        People        Collobration        Publication        Opportunity        Journal Club       


Image:YZ_group.gif

Multi-functional Nanocomposites for Biological Labeling and Diagnostics

There have been tremendous advances in the development of in-situ labeling and screening of different biological entities, ranging from cells to DNAs. Many approaches have been developed for this purpose, such as chemical encoding with molecular tags, organic fluorophores, fluorescent colloids, and Raman fingerprints.

The development of labeling materials has been critically important. Some materials such as quantum nanodots, organic dyes and metal nanoparticles have been extensively used for biological labeling, however, they have to be surface modified to better suit their integration with biological systems. Recently, synthesis of monodisperse polymer nanospheres has stimulated great interest and incorporation of fluorophores in these nanospheres is particularly attractive.

In these nanocomposites, organic polymer can not only stabilize the nanoparticles in a solid matrix, but also effectively combine the peculiar features of organic and inorganic components and thus resulting in novel properties. These materials can bring new and unique capabilities to a variety of biomedical applications ranging from diagnosis of diseases to novel therapies.


Micropatterning of Biomolecules via Manipulation of Micro/Nano Spheres

Micropatterning of biomolecules, the attachment of biomoloecules within designated regions on solid surfaces while preventing nonspecific adhesion at other regions, forms the basis of microarray technnology. It has found many applications in various fields such as the diagnosis of disease, drug discovery, environmental testing, biological studies, etc.

We are developing a new technique of micropatterning which allows the integration of non-planar spots on a planar microarray via anipulation of micro/nano spheres.

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