Cellular and molecular bioengineering laboratory

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Welcome to the Pam Silver lab wiki in [[Main_Page | OPENWETWARE]]
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[[Cellular and Molecular Bioengineering Laboratory: Research| <font face="trebuchet ms" style="color:#000"> '''Research''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:People | <font face="trebuchet ms" style="color:#000"> '''People''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:Collobration | <font face="trebuchet ms" style="color:#000"> '''Collobration ''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:Publication | <font face="trebuchet ms" style="color:#000"> '''Publication''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:Opportunity | <font face="trebuchet ms" style="color:#000"> '''Opportunity ''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Cellular and Molecular Bioengineering Laboratory:Journal Club| <font face="trebuchet ms" style="color:#000"> '''Journal Club ''' </font>]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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== Research Information ==
 
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* [[Silver: Synthetic Biology | Synthetic Biology]]
 
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* [[Silver: Genome Organization | Genome Organization]]
 
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* [[Silver: Pathways in Disease | Pathways  in Disease ]]
 
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* [[Silver: RNA Stuff | RNA Dynamics]]
 
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* [[Silver: Publications | Publications]]
 
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* [[Silver: In the News | In the News]]
 
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*[http://hibie.harvard.edu/ Harvard Institute of Biologically Inspired Engineering]
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[[Image:YZ_group.gif]]
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*[http://www.msi.harvard.edu/ Microbial Sciences Institute]
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*[http://environment.harvard.edu/ Harvard Center for the Environment]
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*[http://www.bionumbers.org/ BioNumbers]
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== Multi-functional Nanocomposites for Biological Labeling and Diagnostics ==
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There have been tremendous advances in the development of in-situ labeling and screening of different biological entities, ranging from cells to DNAs. Many approaches have been developed for this purpose, such as chemical encoding with molecular tags, organic fluorophores, fluorescent colloids, and Raman fingerprints.
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The development of labeling materials has been critically important. Some materials such as quantum nanodots, organic dyes and metal nanoparticles have been extensively used for biological labeling, however, they have to be surface modified to better suit their integration with biological systems. Recently, synthesis of monodisperse polymer nanospheres has stimulated great interest and incorporation of fluorophores in these nanospheres is particularly attractive.  
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In these nanocomposites, organic polymer can not only stabilize the nanoparticles in a solid matrix, but also effectively combine the peculiar features of organic and inorganic components and thus resulting in novel properties. These materials can bring new and unique capabilities to a variety of biomedical applications ranging from diagnosis of diseases to novel therapies.
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==Micropatterning of Biomolecules via Manipulation of Micro/Nano Spheres==
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Micropatterning of biomolecules, the attachment of biomoloecules within designated regions on solid surfaces while preventing nonspecific adhesion at other regions, forms the basis of microarray technnology. It has found many applications in various fields such as the diagnosis of disease, drug discovery, environmental testing, biological studies, etc.  
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== Resources ==
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We are developing a new technique of micropatterning which allows the integration of non-planar spots on a planar microarray via anipulation of micro/nano spheres.
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* [[Silver: Protocols | Protocols]]
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** [[Silver: Protocols#Genome Organization | Genome Organization]]
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** [[Silver: Protocols#RNA .22Stuff.22 | RNA]]
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** [[Silver: Protocols#Synthetic Biology | Synthetic Biology]]
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** [[Silver: Protocols#Cell-Based Screens | Cell-Based Screens ]]
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** [[Silver: Protocols#Methylation | Methylation]]
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** [[Silver: Protocols#Other .2F More General | Other / More General]]
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==Educational Programs==
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*[http://sysbiophd.med.harvard.edu/ Harvard University PhD Program in Systems Biology]
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*[http://www.hms.harvard.edu/dms/bbs/ Biological and Biomedical Sciences Graduate Program]
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*[http://arep.med.harvard.edu/biophysics/ Harvard University Biophysics Program]
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*[http://chembio.med.harvard.edu/ Harvard Chemical Biology Graduate Program]
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*[http://openwetware.org/wiki/IGEM:Harvard/2008 Harvard University iGEM team]
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*[http://bbf.openwetware.org/ The Biobricks Foundation]
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== Lab Information ==
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* [[Silver: Lab Members | Lab Members]]
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* [[Silver: Lab Meeting Shedule | Lab Meeting Schedule]]
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* [[Silver: Former Lab Members | Former Lab Members]]
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* [[Silver: Contact Information | Contact, Strain Requests and Joining the Group]]
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* [[Silver: Open Positions| Open Positions]]
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* [https://silver.med.harvard.edu/passwd/wiki/index.php?title=Main_Page Internal Lab Links (private)]
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**(private link: includes contact info, birthdays and pictures)
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Images on this page provided by Chris Brown and Caroline Ajo-Franklin. 
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Wiki Editing [[Silver: Wiki Instructions|Instructions]]
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Lab members, also visit the [https://silver.med.harvard.edu/passwd/wiki/index.php?title=Main_Page secure wiki].
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Current revision

Research        People        Collobration        Publication        Opportunity        Journal Club       


Image:YZ_group.gif

Multi-functional Nanocomposites for Biological Labeling and Diagnostics

There have been tremendous advances in the development of in-situ labeling and screening of different biological entities, ranging from cells to DNAs. Many approaches have been developed for this purpose, such as chemical encoding with molecular tags, organic fluorophores, fluorescent colloids, and Raman fingerprints.

The development of labeling materials has been critically important. Some materials such as quantum nanodots, organic dyes and metal nanoparticles have been extensively used for biological labeling, however, they have to be surface modified to better suit their integration with biological systems. Recently, synthesis of monodisperse polymer nanospheres has stimulated great interest and incorporation of fluorophores in these nanospheres is particularly attractive.

In these nanocomposites, organic polymer can not only stabilize the nanoparticles in a solid matrix, but also effectively combine the peculiar features of organic and inorganic components and thus resulting in novel properties. These materials can bring new and unique capabilities to a variety of biomedical applications ranging from diagnosis of diseases to novel therapies.


Micropatterning of Biomolecules via Manipulation of Micro/Nano Spheres

Micropatterning of biomolecules, the attachment of biomoloecules within designated regions on solid surfaces while preventing nonspecific adhesion at other regions, forms the basis of microarray technnology. It has found many applications in various fields such as the diagnosis of disease, drug discovery, environmental testing, biological studies, etc.

We are developing a new technique of micropatterning which allows the integration of non-planar spots on a planar microarray via anipulation of micro/nano spheres.

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