Cfrench:anthrone
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Assaying polysaccharides with the anthrone assay
Equipment and Instrument
1. 19×150mm glass test tube
2. Cuvettes
3. Glass pipette and pipette aid
4. Spectrometer
5. Chemical hood
Preparation of Reagent and Standard Solution
1. 2% anthrone in ethyl acetate [SAFETY NOTES: Ethyl acetate is highly flammable in presence of open flames and sparks, of heat. Be careful when using it.]