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Assaying polysaccharides with the anthrone assay

Equipment and Instrument

1. 19×150mm glass test tube

2. Cuvettes

3. Glass pipette and pipette aid

4. Spectrometer

5. Chemical hood

Preparation of Reagent and Standard Solution

1. 2% anthrone in ethyl acetate [SAFETY NOTES: Ethyl acetate is highly flammable in presence of open flames and sparks, of heat. Be careful when using it.]

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