Cfrench:primerlist

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(New page: ==Catalog of primers== ===Primers for iGEM2007 flavours/fragrances project=== '''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c '''Purpose''': forward primer for ''ech (...)
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'''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c
'''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c
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*Purpose: forward primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046.
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'''Purpose''': forward primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046.
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*Restriction sites: EcoRI, XbaI
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*Notes: no ribosome binding site included.
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'''Restriction sites''': EcoRI, XbaI
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'''echr1''' ct actagt a tta tta gcg ttt ata ggc ttg cag c
'''echr1''' ct actagt a tta tta gcg ttt ata ggc ttg cag c
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*Purpose: reverse primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046.
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'''Purpose''': reverse primer for ''ech (ferA)'' coding sequence from ''Ps. fluorescens'' NCIMB 9046.
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*Restriction sites: SpeI only.
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*Notes: replaces TGA stop codon with TAA TAA.
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'''Restriction sites''': SpeI only.
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'''Notes''': replaces TGA stop codon with TAA TAA.
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[[French_Lab|Back to Main Page]]
[[French_Lab|Back to Main Page]]

Revision as of 05:37, 23 July 2007

Catalog of primers

Primers for iGEM2007 flavours/fragrances project

echf1 aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c

  • Purpose: forward primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.
  • Restriction sites: EcoRI, XbaI
  • Notes: no ribosome binding site included.

echr1 ct actagt a tta tta gcg ttt ata ggc ttg cag c

  • Purpose: reverse primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.
  • Restriction sites: SpeI only.
  • Notes: replaces TGA stop codon with TAA TAA.

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