Cfrench:primerlist

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Revision as of 03:35, 23 July 2007 by Cfrench (talk | contribs) (New page: ==Catalog of primers== ===Primers for iGEM2007 flavours/fragrances project=== '''echf1''' aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c '''Purpose''': forward primer for ''ech (...)
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Catalog of primers

Primers for iGEM2007 flavours/fragrances project

echf1 aat gaattc gcggccgc t tctag atg agc aaa tac gaa ggt c

Purpose: forward primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.

Restriction sites: EcoRI, XbaI

echr1 ct actagt a tta tta gcg ttt ata ggc ttg cag c

Purpose: reverse primer for ech (ferA) coding sequence from Ps. fluorescens NCIMB 9046.

Restriction sites: SpeI only.

Notes: replaces TGA stop codon with TAA TAA.

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