ChIP/Liver harvest

Overview

This is a protocol for harvesting mouse liver tissue for chromatin immunoprecipitation.

Materials

Buffer A (100mL total)

• 91mL of H2O
  
• 1.5mL of 1M Hepes
  
• 2mL of 3M KCl
  
• 300µL of 5M NaCl
  
• 40µL of 0.5M EDTA
  
• 100µL of 0.5M EGTA
  
• 16.2µL of 2.1M Sucrose
  
• 7.5µL of 2M BME
  
• 5mL of 2.5M Glycine (to make 2.5M stock- 187.5 g/L)
  
  • A stock of Liver Buffer A can be made without BME or Glycine and stored at room temp. Add BME and Glycine before use.

Buffer B (100mL total)

• 1.5mL of 1M Hepes
  
• 2mL of 3M KCl
  
• 300µm of 5M NaCl
  
• 20µm of 0.5M EDTA
  
• 20µm of 0.5M EGTA
  
• 100mL of 2.1M Sucrose
  

Other

• PBS
  
• PBS/1% Formaldehyde
  
• Avertin (Anesthesia)

Notes

• Please contribute user notes!

References

• (Young Lab)

Contact

• cmc