ChIP/Liver harvest

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Contents

Overview

This is a protocol for harvesting mouse liver tissue for chromatin immunoprecipitation.

Materials

Buffer A (100mL total)

  • 91mL of H2O
  • 1.5mL of 1M Hepes
  • 2mL of 3M KCl
  • 300µL of 5M NaCl
  • 40µL of 0.5M EDTA
  • 100µL of 0.5M EGTA
  • 16.2µL of 2.1M Sucrose
  • 7.5µL of 2M BME
  • 5mL of 2.5M Glycine (to make 2.5M stock- 187.5 g/L)
    • A stock of Liver Buffer A can be made without BME or Glycine and stored at room temp. Add BME and Glycine before use.


Buffer B (100mL total)

  • 1.5mL of 1M Hepes
  • 2mL of 3M KCl
  • 300µm of 5M NaCl
  • 20µm of 0.5M EDTA
  • 20µm of 0.5M EGTA
  • 100mL of 2.1M Sucrose


Other

  • PBS
  • PBS/1% Formaldehyde
  • Avertin (Anesthesia)

Procedure

Notes

  • Please contribute user notes!

References

  • (Young Lab)

Contact

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