Chan:Research: Difference between revisions
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Despite the essential nature of centromeres, their DNA sequence and the CENH3 protein evolve rapidly. To study the functional consequences of rapid centromere evolution, we are manipulating CENH3 and centromere DNA in ''Arabidopsis'' and in other plants. In collaboration with [http://korflab.ucdavis.edu/ Ian Korf], we are also using comparative genomics to study how centromere DNA evolution has been constrained by functional demands.<br> | Despite the essential nature of centromeres, their DNA sequence and the CENH3 protein evolve rapidly. To study the functional consequences of rapid centromere evolution, we are manipulating CENH3 and centromere DNA in ''Arabidopsis'' and in other plants. In collaboration with [http://korflab.ucdavis.edu/ Ian Korf], we are also using comparative genomics to study how centromere DNA evolution has been constrained by functional demands.<br> | ||
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'''3) | '''3) Chromosome engineering to create new plant breeding technologies'''<br> | ||
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Haploid plants that are converted back into diploids can greatly [http://www.formula1.com/ accelerate] plant breeding. Such “doubled haploids” produce instant homozygous lines from heterozygous F1s, a process that normally takes 8-10 generations of inbreeding. As described above, we have discovered a simple method for producing haploid plants through seed by manipulating CENH3. Haploids are easily converted to diploids, so ''Arabidopsis'' geneticists can produce large recombinant populations in a single step, or rapidly generate multiple mutants (e.g. 1 out of 256 haploid progeny will contain eight unlinked mutations if one starts with a octuple heterozygote). A detailed protocol for generating ''Arabidopsis'' haploids is available at [http://tinyurl.com/ArabidopsisHaploidProtocol http://tinyurl.com/ArabidopsisHaploidProtocol]<br> | Haploid plants that are converted back into diploids can greatly [http://www.formula1.com/ accelerate] plant breeding. Such “doubled haploids” produce instant homozygous lines from heterozygous F1s, a process that normally takes 8-10 generations of inbreeding. As described above, we have discovered a simple method for producing haploid plants through seed by manipulating CENH3. Haploids are easily converted to diploids, so ''Arabidopsis'' geneticists can produce large recombinant populations in a single step, or rapidly generate multiple mutants (e.g. 1 out of 256 haploid progeny will contain eight unlinked mutations if one starts with a octuple heterozygote). A detailed protocol for generating ''Arabidopsis'' haploids is available at [http://tinyurl.com/ArabidopsisHaploidProtocol http://tinyurl.com/ArabidopsisHaploidProtocol]<br> |
Revision as of 15:40, 29 January 2011
Chan Lab
Department of Plant Biology at UC Davis
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Centromeres: Controllers of Inheritance |