Chang Lab:Notebook/Assessment of Penetration of Biodegradable Nanopolymers into Biofilm/Entry Base: Difference between revisions
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Date: [[19 January 2009]]; | Date: [[19 January 2009]]; | ||
Time: 1100; | |||
Agenda: Culturing of PA01 inoculum. | |||
Date: [[20 January 2009]]; | |||
Time: 1100; | Time: 1100; | ||
Agenda: Culturing of PA01 inoculum. | Agenda: Culturing of PA01 inoculum. | ||
Revision as of 02:39, 21 January 2009
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ProtocolDate: 3 December 2008; Time: 1100; Agenda: Preparation of Ecoli Inoculum Date: 4 December 2008; Time: 0930; Agenda: Quadrant Streaking of Petri Dishes prepared on 3rd December Date: 5 December 2008; Time: 0945; Agenda: Preparation of Ecoli Inoculum and Quadrant Streaking of Petri Dishes Date: 6 December 2008; Time: 0945; Agenda: Store Petri Dishes that were streaked on 5 December in a 4 degrees fridge. Date: 9 December 2008; Time: 0900; Agenda: Mid-Log Phase Determination Date: 13 December 2008; Time: 0915; Agenda: Biofilm formation using M9 and LB Broth. Date: 15 December 2008; Time: 1400; Agenda: Reprepare M9 medium and quantification of biofilm formed Date: 16 December 2008; Time: 0830; Agenda: Biofilm formation at 3 temperatures for 48hr. Date: 17 December 2008; Time: 0830; Agenda: Results of serial plating done on 16 December and sonication done on 15 December. Date: 18 December 2008; Time: 0930; Agenda: Biofilm formation at 3 temperatures for 24hr. Date: 19 December 2008; Time: 0930; Agenda: Quantification of 24hr biofilm at 3 temperatures and results of serial plating done on 18 December. Date: 20 December 2008; Time: 0930; Agenda: Biofilm formation at 3 temperatures for 72hr. Date: 22 December 2008; Time: 0930; Agenda: Culturing of bacteria to grow biofilm the next day. Date: 23 December 2008; Time: 0930; Agenda: Quantification of 72hr biofilm at 3 temperatures and biofilm formation at 21deg and 37deg for 72hr. Date: 26 December 2008; Time: 1400; Agenda: Quantification of 72hr biofilm at 2 temperatures. Date: 27 December 2008; Time: 0930; Agenda: Biofilm formation at 1 temperature for 48hr and 72hr. Date: 29 December 2008; Time: 1400; Agenda: Quantification of 48hr biofilm at 1 temperature. Date: 30 December 2008; Time: 1400; Agenda: Quantification of 72hr biofilm at 1 temperature and biofilm formation at 24hr and 72hr. Date: 31 December 2008; Time: 1400; Agenda: Quantification of 24hr biofilm at 1 temperature. Date: 2 January 2009; Time: 1400; Agenda: Quantification of 72hr biofilm at 1 temperature. Date: 4 January 2009; Time: 1400; Agenda: Streaking of agar plates from slant culture Date: 6 January 2009; Time: 1400; Agenda: Biofilm formation at 1 temperature for 24hr. Date: 7 January 2009; Time: 1400; Agenda: Quantification of 24hr biofilm at 1 temperature and biofilm formation at 1 temperature for 24hr and 48hr. Date: 8 January 2009; Time: 1430; Agenda: Quantification of 24hr and 48hr biofilm at 1 temperature each. Date: 19 January 2009; Time: 1100; Agenda: Culturing of PA01 inoculum. Date: 20 January 2009; Time: 1100; Agenda: Culturing of PA01 inoculum. | |
