Chang Lab:Notebook/CBE/08/146/2008/12/30

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Biofilm quantification of Trial VI (30C, 72h)

  • Took planktonic absorbance reading
  • Plated 10^-2, 10^-4, 10^-6 of planktonic from wells, D4 (for M9) and D9 (for LB).

Biofilm optimization Trial VII

  • 30C, 24 and 72h.
    • Using new colorless M9 (did not autoclave after mixing with M9 salt this time round)
    • Column 1-6: M9
    • Column 7-12: LB

Plate count of 29/12 planktonic (Trial VI- 48h, 30C)

  • LB
    • 10^-6 - 492, 3)
    • 10^-8 - 33, 2)
    • 10^-10- 6, -)
  • M9
    • 10^-6 - 3
    • 10^-8 - 2
    • 10^-10- none


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