Chang Lab:Notebook/CBE/08/149/2008/12/15: Difference between revisions
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===New M9 medium protocol=== | ===New M9 medium protocol=== | ||
* In 1L of volumetric flask, the following components were weighed using a weighing machine and added into the flask: | * In 1L of volumetric flask, the following components were weighed using a weighing machine and added into the flask: | ||
**Na2HPO4 (10g) | |||
**KH2PO4 (5g) | |||
**NH4Cl (5g) | |||
**NaCl (2.5g) | |||
* This 1L 5X concentrate of M9 minimal salts is dissolved in 1L of distilled water. | |||
** The flask is autoclaved for 15minutes at 121 degrees. | ** The flask is autoclaved for 15minutes at 121 degrees. | ||
** 200ml of this sterile 5X M9 Minimal Salt solution is added to 750ml sterile distilled water. | ** 200ml of this sterile 5X M9 Minimal Salt solution is added to 750ml sterile distilled water. |
Latest revision as of 19:52, 26 December 2008
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LogsDate: 15 December 2008; Time: 1400; Agenda: Re-prepare M9 medium and quantification of biofilm formed.
New M9 medium protocol
Biofilm Preparation (CV staining and Sonication) - Trial I
Biofilm Quantification
Protocol for CV staining (half of 96 wells = 48 wells)
Protocol for sonication (other half of 96 wells = 48 wells)
This marks the end of quantification of biofilm. Notes
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