Chang Lab:Notebook/CBE/08/149/2008/12/30: Difference between revisions

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===Biofilm optimization Trial VII===
===Biofilm optimization Trial VII===
* CONDITIONS: 30C, 24 and 48h.
* CONDITIONS: 30C, 24 and 72h.
* <b> USING NEW COLORLESS M9 </b>
* <b> USING NEW COLORLESS M9 </b>
* Column 1-6: M9
* Column 1-6: M9

Latest revision as of 02:33, 4 January 2009

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Antimicrobial efficacy testing of antibiotic-containing biodegradable nanopolymers against biofilm and planktonic cells
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Logs

Biofilm quantification of Trial VI (30C, 72h)

  • Took planktonic absorbance reading
  • Plated 10^-2, 10^-4, 10^-6 of planktonic from wells, D4 (for M9) and D9 (for LB).

Biofilm optimization Trial VII

  • CONDITIONS: 30C, 24 and 72h.
  • USING NEW COLORLESS M9
  • Column 1-6: M9
  • Column 7-12: LB

Plate count of 12/29 planktonic (TVI-48h-30C)

  • (LB, M9)
  • 10^-6: (492, 3)
  • 10^-8: (33, 2)
  • 10^-10: (6, -)



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