Chassis/System - BC Box 1: Difference between revisions
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| || || [[VM#VM1.0#Introduction|BL21(VM1.0)]] || pSB4A3.MG1655 || BL21(DE3) from SK; 7/28/05 || D1210 || DH5α || MC4100 || JM109 || MG1655 | | || || [[VM#VM1.0#Introduction|BL21(VM1.0)]] || pSB4A3.MG1655 || BL21(DE3) from SK; 7/28/05 || D1210 || DH5α || MC4100 || JM109 || MG1655 | ||
|} | |} | ||
{| border="1" cellpadding="2" | |||
|+BC Construction Parts | |||
|- | |||
! 1 | |||
| || || ||E7104.1A2.BL21(DE3) 10/8 ||E7105.1A2.BL21(DE3) 10/27 ||E7107.1A2.BL21(DE3) 10/27 ||E7108.1A2.BL21(DE3) 10/27 ||E70202.1A3.TOP10 9/13 ||E71208.1A2.TOP10 1/5/07 | |||
|- | |||
! 10 | |||
|E71214.1A2.TOP10 1/5/07 ||8.mCherry.E70202.1A3.TOP10 2/15 ||2.8 mCherry.E70202.1A2.tOP10 2/15 ||4B.mCherry.E70202.1A3.TOP10 2/15 || ||4B.mCHerry.E7108.1A3.TOP10 2/15||8.mCherry.E7108.1A3.TOP10 2/15 ||2.8mCherry.E7108.1A3.TOP10 2/15 ||4B.mCherry.E71205.1AK3.TOP10 2/16 | |||
|- | |||
! 19 | |||
|8mCherry.E71205.1AK3.TOP10 2/16 ||2.8mCherry.E71205.1AK3.TOP10 2/16 ||4B.mCherry.E7108.1AK3.TOP10 2/16 ||4B.mCherry.I7101.1AK3.TOP10 2/16 ||8mCherry.I7101.1AK3.TOP10 2/16 ||2.8mCherry.I7101.1AK3.TOP10 2/16 ||rRNA-4(fixed).pCH1497.TOP10 2/16 ||4BmCherry.E70202.1AK3.BL21(VM1.1) 2/18 || | |||
|- | |||
! 28 | |||
|8mCherry.E70202.1AK3.BL21(VM1.1) 2/18 ||2.8mCherry.E70202.1AK3.BL21(VM1.1 2/18 ||4BmCherry.E7108.1AK3.BL21(VM1.1) 2/18||8mCherry.E7108.1AK3.BL21(VM1.1) 2/18 ||2.8mCherry.E7108.1AK3.BL21(VM1.1) 2/18 ||4B.mCherry.E71205.1AK3.BL21(VM1.1) 2/18 ||8mCherry.E71205.1AK3.BL21(VM1.1) 2/18 ||2.8mCherry.E71205.1AK3.BL21(VM1.1) 2/18 ||4B.mCherry.I7101.1AK3.BL21(VM1.1) 2/18 | |||
|- | |||
! 37 | |||
|8mCherry.I7101.1AK3.BL21(VM1.1) 2/18 ||2.8mCherry.I7101.1AK3.BL21(VM1.1) 2/18 ||R0184.1Ak3.TOP10 5/8/07 ||C0013.1AK3.TOP10 5/12/07 ||I2032.1AT3.TOP10 5/16/07 ||S03690.1AT3.TOP10 5/16/07 ||S03691.1AT3.TOP10 5/16/07 ||S03691.1AT3.TOP10 (2) 5/16/07 ||S03692.1AT3.TOP10 5/18/07 | |||
|- | |||
! 46 | |||
|B0073.1AT3.TOP10 5/18/07 ||8mCherry.I7101.1AK3.TOP10 5/18/07 ||8mCherry.I7101.1AK3.TOP10 (2) 5/18/07 ||B0074.1AK3.TOP10 5/18/08 ||S03704.1AC3.TOP10 5/20/07 ||I2034.1AC3.TOP10 5/20 ||I2035.1AT3.TOP10 5/20/07 ||R4032.1AT3.TOP10 5/20/07 ||I2032.1AK3.TOP10 5/20/07 | |||
|- | |||
! 55 | |||
|S03693.1AT3.TOP10 5/25 ||I2032.1AK3.TOP10 (2) 5/25/07 ||S03705.1AC3.TOP10 5/25/07 ||S03703.1AT3.TOP10 5/28/07 ||I2043.1AT3.TOP10 6/4/07 ||I2043.1AT3.TOP10 6/4/07 ||I2044.1AK3.TOP10 6/4/07 ||I2044.1AK3.TOP10 6/4/07 ||I2041.1AC3.TOP10 5/20/07 | |||
|- | |||
! 64 | |||
|I2041.1AC3.TOP10 6/15/07 ||I2048.4T5.TOP10 6/15/07 ||I2045.3k3.TOP10 6/15/07 ||I2046.3k3.TOP10 6/15/07 ||I2047.3k3.TOP10 6/14/07 ||I2048.4T5+I2035.1AT3.TOP10 6/18/07 ||I2048.4T5+E7108.1A2.TOP10 6/18/07 ||E7108.1A2 TOP10 7/20.07 || | |||
|- | |||
! 73 | |||
|I2032-1.1AK3.TOP10 7/13/07||I2032-2.1AK3.TOP10 7/13/07||I20241.1AK3.TOP10 7/23||I2041.1AC3+S03703.B0016.TOP10 7/23||I2041.1AC3+B0032.S03693.TOP10 7/23/07||S03703.1AT3.TOP10 7/24/07||S03693.1AT3.TOP10 7/24/07||S03746.1AC3.TOP10 7/24/07||I2043.3k3.TOP10 (1) 8/21/07) | |||
|} | |||
{| border="1" cellpadding="2" | {| border="1" cellpadding="2" | ||
Revision as of 17:12, 24 August 2008
| 1 | (pCH1497+I7102.4A3).MG1655 5/4/05 | R00101.E0040.4A3.MG1655 5/31/05 | I7102.3k3.MG1655 4/21/05 | I13401.1A2.? 4/11/05 | pCH1497.MG1655 4/24/05 | Tφ.?A?.? 5/31 | I7101.4A3.MG1655; 7/22/05 | I7102.4A3.MG1655; 7/22/05 | ||
|---|---|---|---|---|---|---|---|---|---|---|
| 11 | E7104.1A2.BL21(DE3) 10/8/05 | (I7103.1A2+pCH1497).BL21 8/30/05 | E7103.1A2.BL21(DE3?) 9/2/05 | E7103.1A2.BL21(DE3) 9/4/05 | E7103.1A3.BL21(DE3) 12/7/05 | E7103.1A2.DH5α 6/20/05 | ||||
| 21 | φ10-YFP.pSB1A2 SK | E7104.1A3.BL21(VM1.0) 10/10/05 | I7101.1A2.BL21(VM1.0) 10/25/05 | E7104.1A2.BL21(DE3) | E7105.1A2.BL21(DE3) | E7107.1A2.BL21(DE3) | E7108.1A2.BL21(DE3) | |||
| 31 | φ10 SK | E0040.B0016.1A2.DH5α? 12/7/05 | ||||||||
| 41 | φ10-CFP.pSB1A2 SK | E70101.1A3.DH5α 2/7/06 | ||||||||
| 51 | φ10-YFP.pSB1A2 SK | |||||||||
| 61 | pBAD.gene1 SK | |||||||||
| 71 | Tφ.3 SK | I7101.1A2.BL21(DE3) 9/4/05 | ||||||||
| 81 | B0015.φ10-YFP SK | pSB3k1.? from RS; 7/1/05 | pSB3k3-1.DB3.1 from RS; 6/30/05 | |||||||
| 91 | BL21(VM1.0) | pSB4A3.MG1655 | BL21(DE3) from SK; 7/28/05 | D1210 | DH5α | MC4100 | JM109 | MG1655 |
| 1 | E7104.1A2.BL21(DE3) 10/8 | E7105.1A2.BL21(DE3) 10/27 | E7107.1A2.BL21(DE3) 10/27 | E7108.1A2.BL21(DE3) 10/27 | E70202.1A3.TOP10 9/13 | E71208.1A2.TOP10 1/5/07 | |||
|---|---|---|---|---|---|---|---|---|---|
| 10 | E71214.1A2.TOP10 1/5/07 | 8.mCherry.E70202.1A3.TOP10 2/15 | 2.8 mCherry.E70202.1A2.tOP10 2/15 | 4B.mCherry.E70202.1A3.TOP10 2/15 | 4B.mCHerry.E7108.1A3.TOP10 2/15 | 8.mCherry.E7108.1A3.TOP10 2/15 | 2.8mCherry.E7108.1A3.TOP10 2/15 | 4B.mCherry.E71205.1AK3.TOP10 2/16 | |
| 19 | 8mCherry.E71205.1AK3.TOP10 2/16 | 2.8mCherry.E71205.1AK3.TOP10 2/16 | 4B.mCherry.E7108.1AK3.TOP10 2/16 | 4B.mCherry.I7101.1AK3.TOP10 2/16 | 8mCherry.I7101.1AK3.TOP10 2/16 | 2.8mCherry.I7101.1AK3.TOP10 2/16 | rRNA-4(fixed).pCH1497.TOP10 2/16 | 4BmCherry.E70202.1AK3.BL21(VM1.1) 2/18 | |
| 28 | 8mCherry.E70202.1AK3.BL21(VM1.1) 2/18 | 2.8mCherry.E70202.1AK3.BL21(VM1.1 2/18 | 4BmCherry.E7108.1AK3.BL21(VM1.1) 2/18 | 8mCherry.E7108.1AK3.BL21(VM1.1) 2/18 | 2.8mCherry.E7108.1AK3.BL21(VM1.1) 2/18 | 4B.mCherry.E71205.1AK3.BL21(VM1.1) 2/18 | 8mCherry.E71205.1AK3.BL21(VM1.1) 2/18 | 2.8mCherry.E71205.1AK3.BL21(VM1.1) 2/18 | 4B.mCherry.I7101.1AK3.BL21(VM1.1) 2/18 |
| 37 | 8mCherry.I7101.1AK3.BL21(VM1.1) 2/18 | 2.8mCherry.I7101.1AK3.BL21(VM1.1) 2/18 | R0184.1Ak3.TOP10 5/8/07 | C0013.1AK3.TOP10 5/12/07 | I2032.1AT3.TOP10 5/16/07 | S03690.1AT3.TOP10 5/16/07 | S03691.1AT3.TOP10 5/16/07 | S03691.1AT3.TOP10 (2) 5/16/07 | S03692.1AT3.TOP10 5/18/07 |
| 46 | B0073.1AT3.TOP10 5/18/07 | 8mCherry.I7101.1AK3.TOP10 5/18/07 | 8mCherry.I7101.1AK3.TOP10 (2) 5/18/07 | B0074.1AK3.TOP10 5/18/08 | S03704.1AC3.TOP10 5/20/07 | I2034.1AC3.TOP10 5/20 | I2035.1AT3.TOP10 5/20/07 | R4032.1AT3.TOP10 5/20/07 | I2032.1AK3.TOP10 5/20/07 |
| 55 | S03693.1AT3.TOP10 5/25 | I2032.1AK3.TOP10 (2) 5/25/07 | S03705.1AC3.TOP10 5/25/07 | S03703.1AT3.TOP10 5/28/07 | I2043.1AT3.TOP10 6/4/07 | I2043.1AT3.TOP10 6/4/07 | I2044.1AK3.TOP10 6/4/07 | I2044.1AK3.TOP10 6/4/07 | I2041.1AC3.TOP10 5/20/07 |
| 64 | I2041.1AC3.TOP10 6/15/07 | I2048.4T5.TOP10 6/15/07 | I2045.3k3.TOP10 6/15/07 | I2046.3k3.TOP10 6/15/07 | I2047.3k3.TOP10 6/14/07 | I2048.4T5+I2035.1AT3.TOP10 6/18/07 | I2048.4T5+E7108.1A2.TOP10 6/18/07 | E7108.1A2 TOP10 7/20.07 | |
| 73 | I2032-1.1AK3.TOP10 7/13/07 | I2032-2.1AK3.TOP10 7/13/07 | I20241.1AK3.TOP10 7/23 | I2041.1AC3+S03703.B0016.TOP10 7/23 | I2041.1AC3+B0032.S03693.TOP10 7/23/07 | S03703.1AT3.TOP10 7/24/07 | S03693.1AT3.TOP10 7/24/07 | S03746.1AC3.TOP10 7/24/07 | I2043.3k3.TOP10 (1) 8/21/07) |
| 1 | I2053.4T5.TOP10 7/30/07 | I20243.4T5.TOP10 7/30/07 | I2044.1AK3.TOP10 7/30/07 | I20243.4T5+E7107.1A2.TOP10 7/31/07 | I20243.4C5+I2035.1AT3.TOP10 7/31/07 | I20243.4C5+E7107.1A2.TOP10 7/31/07 | I20243.4C5.TOP10 7/30/07 | I20243.4C5.TOP 8/6/07 | ||
|---|---|---|---|---|---|---|---|---|---|---|
| 10 | C7053.1AK3.TOP10 8/6/07 | I20257.4C5.TOP10 129/07 | pLysS (CamR).TOP10 8/22/07 | I20243.4C5.D1210 12/1/07 | S03908.1AC3.TOP10 (2) 12/1/07 | S03908.1AC3.TOP10 (3) 12/1/07 | S03909.1AC3.TOP10 (2) 12/1/07 | S03909.1AC3.tOP10 (3) 12/1/07 | S03910.1AC3.TOP10 12/1/07 | |
| 19 | S03911.1AC3.TOP10 12/1/07 | S03914.1AT3.TOP10 12/6/07 | I20253.1AK3.TOP10 12/6/07 | I20253.1AT3.TOP10 12/6/07 | I20257.1AK3.TOP10 (16) 12/7/07 | I20254.1AT3.TOP10 12/7/07 | I20257.1AC3.TOP10 (1) 12/9/07 | I20257.4C5+pCH1497-rRNA-4.TOP10 12/15/07 | ||
| 28 | I20257.4C5+E71205.1A3.TOP10 12/15/07 | I20257.4C5+rRNA-4.pCH1497+E71205.1A3.TOP10 12/15/07 | I20257.4C5+PCH1497.rRNA-4+I7101.1A3.TOP10 12/16/07 | pCH1497-rRNA-4+E71205.1A3.TOP10 1/6/08 | I0500.2K3.D1210 1/13/08 | R0184.rRNA-4move2.2K3.TOP10 1/13/08 | R0184.rRNA-4move2.2K3.TOP10 1/13/08 | PET14b XL1 Blue (from Bijou Bose) 1/19/08 | S03920.1AT3.TOP10 1/19/08 | |
| 37 | E70202.1Ae+pCH1497-rRNA-4Fixed.TOP10 2/17/08 | T7 pCH1497-rRNA-4 Lig2 | R0183.rRNA-4.1AK3.TOP10 (2) 2/24/08 | R0183.rRNA-4.1AK3.TOP10 (3) 2/24/08 | R0183.rRNA-4.2K3.TOP10 2/26/08 | R0183.rRNA-4.1AK3+I20257.4C5.TOP10 3/9/07 | R0183.rRNA-4.2K3+I20257.4C5+E71205.1A3.TOP10 3/9/08 | R0183.rRNA-4.2K3+E71205.1A3.TOP10 3/9/08 | ||
| 46 | R0183.rRNA-4.1AK3.BL21(DE3) 3/9/08 | R0183.rRNA-4.2K3+I20257.4C5.TOP10 3/9/08 | E70202.1A3.BL21(DE3) 3/9/08 | R0183.rRNA-4.2K3+E71205.1A3.BL21(DE3) 3/9/08 | R0183.rRNA-4.pCH1497+E70202.1A3.BL21(DE3) (1) 3/9/08 | R0183.rRNA-4.pCH1497.BL21(DE3) 3/9/08 | R0183.rRNA-4.pCH1497+E70202.BL21(DE3) (1) 3/9/08 | R0184.rRNA-4.pCH1497.BL21(E3) 3/28/08 | ||
| 55 | R0184.rRNA-4.pCH1497+E70202.1A3.BL21(DE3) 3/28/08 | R0183.rRNA-4.pCH1497.+E71205.1A3.BL21(DE3) 3/10/08 | R0183.rRNA-4.pCH1497.+E70202.1A3.BL21(DE3) (2) 3/9/08 | R0183.rRNA-4.pCH1497+E70202.1A3.BL21(DE3) (3) 3/9/08 | R0183.rRNA-4.2K3.BL21(DE3) 3/9/08 | E70202.1A3+R0183.rRNA-4.2K3.BL21(DE3) (2) 3/9/08 | R0184.rRNA-4.pCh1497 TOP10 (2-1) 3/20/08 | R0184.rRNA-4.pCH1497+E71205.1A3.TOP10 3/28/08 | R0184.rRNA-4.pCH1497+E71205.1A3.TOP10 4/20/08 | |
| 64 | R0184.rRNA-4.pCH1497+E70202.1A3.BL21(AI) 3/28/08 | I20257.R0184.rRNA-4.4C5.TOP10 3/28/08 | I20257.R0184.rRNA-4.4K5.TOP10 3/28/08 | R0184.rRNA-4.pCH1497+I20257.4C5.D1210 (1) 3/28/08 | R0184.rRNA-4pCH1497+I20257.4C5.D1210 (2) 3/28/08 | E71205.1A3.D1210 3/29/08 | R0184.rRNA-4.pCH1497+E71205.1A3.D1210 3/29/08 | I20257.4C5+E71205.1A3.D1210 3/29/08 | ||
| 73 | R0184.rRNA-4.pCH1497+I20257.4C5+E71205.1A3.D1210 (1) 3/29/08 | R0184.rRNA-4.pCH1497+I20257.4C5+E71205.1A3.D1210 (2) 3/29/08 | R0184.rRNA-4.pCH1497+I20257.4C5+pUC19.D1210 (1) 3/29/08 | R0184.rRNA-4.pCH1497+I20257.4C5+pUC19.D1210 (2) 3/29/08 | R0185-6.TOP10 | R018x-1.TOP10 | R018x-4.TOP10 | - |
| 1 | B0032.C7053-GFP.1AT3.TOP102/18/08 | B0032.C7077-GFP.1AT3.TOP103/12/08 | B0033.C7053-GFP.1AT3.TOP103/11/08 | B0033.C7077-GFP.1AT3.TOP103/11/08 | B0073.C7053-GFP.1AT3.TOP103/11/08 | B0073.C7077-GFP.1AT3.TOP103/11/08 | B0075.C7053-GFP.1AT3.TOP102/18/08 | B0075.C7053-GFP.1AT3.TOP103/11/08 | I20271.4C5.D1210 4/8/08 | |
|---|---|---|---|---|---|---|---|---|---|---|
| 10 | I20271.4C5.D1210 (2) 4/8/08 | I20272.1AT3.TOP10 4/8/08 | I20274.1AT3.TOP10 4/8/08 | I20275.1AT3.TOP10 4/8/08 | R0185.rRNA-4.pCH1497.BL21(DE3) 4/8/08 | R0186.rRNA-4.pCH1497.BL21(DE3) 4/8/08 | R0186.rRNA-4.pCH1497.BL21(DE3) + E70202.1A3 4/8/08 | R0187.rRNA-4.pCH1497.BL21(DE3) 4/8/08 | R0187.rRNA-4.pCH1497.BL21(DE3) + E70202.1A3 4/8/08 | |
| 19 | I20273.1AC3.TOP10 4/10/08 | I20276.4K5.TOP10 4/12/08 | I20278.4K5.TOP10 4/12/08 | S03937.4K5.TOP10 4/12/08 | S03937.1AK3.TOP10 4/12/08 | S03938.4K5.TOP10 4/12/08 | I20279.1AT3.TOP10 4/12/08 | |||
| 28 | R0185.rRNA-4.pCH1497 E70202.1A2 BL21(DE3) 4/14/08 | I20280.4C5.TOP10 4/16/08 | I20281.4C5.TOP10 4/16/08 | I20279.4C5.TOP10 4/15/08 | I20279.4C5+pBAD.rRNA-4.pCH1497.TOP10 4/19/08 | I20280.4C5+pBAD.rRNA-4.pCH1497.TOP10 4/18/08 | I20280.4C5+R0184.rRNA-4.pCH1497.TOP10 4/18/08 | I20280.4C5+R0185.rRNA-4.pCH1497.TOP10 4/18/08 | ||
| 37 | I20280.4C5+R0186.rRNA-4.pCH1497.TOP10 4/19/08 | I20280.4C5+R0187.rRNA-4.pCH1497.TOP10 4/19/08 | I20281.4C5+pBAD.rRNA-4.pCH1497.TOP10 4/19/08 | I20281.4C5+R0184.rRNA-4.pCH1497.TOP10 4/19/08 | I20281.4C5+R0185.rRNA-4.pCH1497.TOP10 4/19/08 | I20281.4C5+R0186.rRNA-4.pCH1497.TOP10 4/19/08 | I20281.4C5+R0187.rRNA-4.pCH1497.TOP10 4/19/08 | I20281.4C5+R0185.rRNA-4.pCH1497+E71205.1A3.TOP10 4/20/08 | I20281.4C5+R0185.rRNA-4.pCH1497+pUC19.TOP10 4/20/08 | |
| 46 | I20281.4C5+E71205.1A3.TOP10 4/20/08 | R0185.rRNA-4.pCH1497+E71205.1A3.TOP10 4/20/08 | I20281.4C5+R0184.rRNA-4.pCH1497+E71205.1A3.TOP10 4/20/08 | I20281.4C5+R0184.rRNA-4.pCH1497.TOP10 4/20/08 | I20280.4C5+E71205.1A3.TOP10 4/20/08 | E71205.1A3.TOP10 4/21/08 | R0186.rRNA-4.pCH1497+E71205.1A3.TOP10 4/21/08 | I20279.4C5+E71205.1A3.TOP10 4/21/08 | ||
| 55 | I20280.4C5+R0184.rRNA-4.pCH1497.pUC19.TOP10 4/21/08 | I20280.4C5+R0184.rRNA-4.pCH1497+E71205.1A3.TOP10 4/21/08 | I20280.1A3+R0186.rRNA-4.pCH1497+pUC19.TOP10 4/21/08 | I20280.4C5+R0186.rRNA-4.pCH1497+E71205.1A3.TOP10 4/21/08 | I20280.4C5+R0185.rRNA-4.CH1497+E71205.1A3.TOP10 4/21/08 | I20279.4C5+pBAD.rRNA-4.pCH1497+E70202.1A3.TOP10 4/21/08 | I20279.4C5+pBAD.rRNA-4.pCH1497+E71205.1A3.TOP10 4/22/08 | I20279.4C5+pBAD.rRNA-4.pCH1497+I7101.1A3.TOP10 4/22/08 | I20279.4C5+pBAD.rRNA-4.pCH1497+pUC19.TOP10 4/22/08 | |
| 64 | R0185.rRNA-4.pCH1497+E70202.1A3.TOP10 4/22/08 | I20280.4C5+R0184.rRNA-4.pCH1497+E70202.1A3.TOP10 4/22/08 | I20280.4C5+R0185.rRNA-4.pCH1497+E70202.1A3.TOP10 4/22/08 | I20281.4C5+R0185.rRNA-4.pCH1497+E70202.1A3.TOP10 4/22/08 | I20280.4C5+R0185.rRNA-4.pCH1497+pUC19.TOP10 4/22/08 | I20281.4C5+R0184.rRNA-4.pCH1497+E70202.1A3.TOP10 4/22/08 | R0184.rRNA-4.pCH1497+E70202.1A3.TOP10 4/22/08 | I20279.4C5+pBAD.rRNA-4.pCH1497+E7108.4C5.TOP10 4/22/08 | I20281.4C5+R0184.rRNA-4.pCH1497+E7108.1A3.TOP10 4/22/08 | |
| 73 | I20281.4C5+E7108.TOP10 4/22/08 | SQ351 MG 4/23/08 | SQ2158 MG 4/23/08 | S27 MG 4/23/08 | I20280.4C5+R0184.rRNA-4.pCH1497+E71205.1A3.TOP10 4/28/08 | I20280.4C5+R0184.rRNA-4.pCH1497+pUC19.TOP10 4/26/08 | I20281.4C5+R0186.rRNA-4.pCH1497+pUC19.TOP10 4/26/08 | I20281.4C5+R0186.rRNA-4.pCH1497+E71205.1A3.TOP10 4/26/08 | I2035.1AT3+I2043.3k3.BL21(AI) 4/29/08 |
