Chemical transformation buffer comparison: Difference between revisions

Revision as of 16:03, 30 April 2006

Buffer KCl KCH3COO CaCl2 MnCl2 RbCl Glycerol HACoCl3 DMSO Sucrose pH Ref

SF 100uM 10uM 50uM 10% 6.1 Hanahan patent RFT 30uM 10uM 50uM 100uM 15% 5.8 Hanahan patent FSB 100mM 10mM 45mM 10% w/v 3mM yes 6.2 Hanahan patent

FSB 100mM 10mM 10mM 44mM ? 10% 3mM 2x 3.5% 5% 6.4 -> 6.2 Jesse patent FSB 100mM 10mM 10mM 45mM 8% 3mM 2x 3.5% 5% 6.4 Invitrogen '525

   (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)

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 FSB        100mM  10mM                       45mM                  10% w/v     3mM         yes                             6.2        Hanahan patent
-FSB         100mM  10mM        10mM     44mM     ?            10%           3mM        3.5x2%   5%                6.4 -> 6.2  Jesse patent
+FSB        100mM  10mM        10mM     44mM     ?            10%           3mM        2x 3.5%   5%                6.4 -> 6.2  Jesse patent
+FSB        100mM  10mM        10mM      45mM                   8%            3mM        2x 3.5%   5%                6.4         Invitrogen '525
+    (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)