Chemical transformation buffer comparison: Difference between revisions
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FSB 100mM 10mM 10mM 45mM 8% 3mM 2x 3.5% 5% 6.4 Invitrogen '525 | FSB 100mM 10mM 10mM 45mM 8% 3mM 2x 3.5% 5% 6.4 Invitrogen '525 | ||
(add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock) | (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock) | ||
CCMB80 10mM 80mM 20mM 10% 6.4 Invitrogen '464 (10 mM MgCl2) | |||
Revision as of 20:35, 30 April 2006
Buffer KCl KCH3COO CaCl2 MnCl2 RbCl Glycerol HACoCl3 DMSO Sucrose pH Ref
SF 100uM 10uM 50uM 10% 6.1 Hanahan patent RFT 30uM 10uM 50uM 100uM 15% 5.8 Hanahan patent FSB 100mM 10mM 45mM 10% w/v 3mM yes 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 10% 3mM 2x 3.5% 5% 6.4 -> 6.2 Jesse patent FSB 100mM 10mM 10mM 45mM 8% 3mM 2x 3.5% 5% 6.4 Invitrogen '525
(add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)
CCMB80 10mM 80mM 20mM 10% 6.4 Invitrogen '464 (10 mM MgCl2)
