Chemical transformation buffer comparison

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     (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)
     (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)
CCMB80              10mM        80mM    20mM                  10%                                                              6.4        Invitrogen '464 (10 mM MgCl2)
CCMB80              10mM        80mM    20mM                  10%                                                              6.4        Invitrogen '464 (10 mM MgCl2)
 +
TB          250mM                    15mM    15mM                                                  7%                              6.7 -> 6.2  Inoue

Revision as of 19:33, 1 May 2006

Buffer KCl KCH3COO CaCl2 MnCl2 RbCl Glycerol HACoCl3 DMSO Sucrose pH Ref

SF 100uM 10uM 50uM 10% 6.1 Hanahan patent RFT 30uM 10uM 50uM 100uM 15% 5.8 Hanahan patent FSB 100mM 10mM 45mM 10% w/v 3mM yes 6.2 Hanahan patent

FSB 100mM 10mM 10mM 44mM  ? 10% 3mM 2x 3.5% 5% 6.4 -> 6.2 Jesse patent FSB 100mM 10mM 10mM 45mM 8% 3mM 2x 3.5% 5% 6.4 Invitrogen '525

   (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)

CCMB80 10mM 80mM 20mM 10% 6.4 Invitrogen '464 (10 mM MgCl2) TB 250mM 15mM 15mM 7% 6.7 -> 6.2 Inoue

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