Chemical transformation buffer comparison

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Buffer   KCl       KCH3COO    CaCl2      MnCl2    RbCl     Glycerol   HACoCl3  DMSO     Sucrose         pH       Ref
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{| border="1"
-
+
|-
-
SF         100uM  10uM          50uM                                10%                                                               6.1       Hanahan patent
+
|'''Buffer'''
-
RFT                     30uM          10uM      50uM      100uM  15%                                                             5.8       Hanahan patent
+
|'''KCl'''
-
FSB       100mM 10mM                       45mM                 10% w/v     3mM        yes                            6.2       Hanahan patent
+
|'''KCH<sub>3</sub>COO'''
 +
|'''CaCl<sub>2</sub>'''
 +
|'''MnCl<sub>2</sub>'''
 +
|'''RbCl'''
 +
|'''HACoCl<sub>3</sub>'''
 +
|'''Glycerol'''
 +
|'''DMSO'''
 +
|'''Sucrose'''
 +
|'''Other'''
 +
|'''pH'''
 +
|'''Reference'''
 +
|-
 +
|SF
 +
|100mM
 +
| 10mM
 +
| 50mM
 +
|
 +
|
 +
|
 +
|10%
 +
|
 +
|
 +
|
 +
|pH 6.1
 +
|Hanahan patent
 +
|-
 +
|RFT
 +
|
 +
| 30mM
 +
| 10mM
 +
|50mM
 +
|100mM
 +
|
 +
|15%
 +
|
 +
|
 +
|
 +
|pH 5.8
 +
|Hanahan patent
 +
|-
 +
|FSB
 +
|100mM
 +
|10mM
 +
|
 +
|45mM
 +
|
 +
|3mM
 +
|10% w/v
 +
|
 +
|
 +
|
 +
|pH 6.2
 +
|Hanahan patent
 +
|-
 +
|FSB
 +
|100mM
 +
|10mM
 +
|10mM
 +
|44mM
 +
|?
 +
|3mM
 +
|10%
 +
|2x 3.5%
 +
|5%
 +
|
 +
|pH 6.4->6.2
 +
|Jessee patent
-
FSB       100mM 10mM       10mM     44mM    ?            10%          3mM       2x 3.5%   5%               6.4 -> 6.2 Jesse patent
+
|-
-
FSB        100mM  10mM        10mM      45mM                  8%            3mM        2x 3.5%  5%                6.4        Invitrogen '525
+
|FSB
-
    (add 50 uM b-ME and 110mM NaCl, incubate on ice for 10 min, add DNA, 1/2 hr on ice, heat shock)
+
|100mM
-
CCMB80             10mM         80mM     20mM                 10%                                                             6.4         Invitrogen '464 (10 mM MgCl2)
+
|10mM
-
TB         250mM                     15mM     15mM                                                 7%                               6.7 -> 6.2   Inoue
+
|10mM
 +
|45mM
 +
|
 +
|3mM
 +
|8%
 +
|2x 3.5%
 +
|5%
 +
|
 +
|pH 6.4->6.2
 +
|Invitrogen '525
 +
|-
 +
|CCMB80
 +
|
 +
|10mM
 +
|80mM
 +
|20mM
 +
|
 +
|
 +
|10%
 +
|7%
 +
|
 +
|
 +
|pH 6.4
 +
|Invitrogen '464
 +
|-
 +
|TB
 +
|250mM
 +
|
 +
|15mM
 +
|15mM
 +
|
 +
|
 +
|
 +
|7%
 +
|
 +
|
 +
|pH 5.7->6.2
 +
|Inoue90
 +
|}

Revision as of 13:22, 2 May 2006

Buffer KCl KCH3COO CaCl2 MnCl2 RbCl HACoCl3 Glycerol DMSO Sucrose Other pH Reference
SF 100mM 10mM 50mM 10% pH 6.1 Hanahan patent
RFT 30mM 10mM 50mM 100mM 15% pH 5.8 Hanahan patent
FSB 100mM 10mM 45mM 3mM 10% w/v pH 6.2 Hanahan patent
FSB 100mM 10mM 10mM 44mM ? 3mM 10% 2x 3.5% 5% pH 6.4->6.2 Jessee patent
FSB 100mM 10mM 10mM 45mM 3mM 8% 2x 3.5% 5% pH 6.4->6.2 Invitrogen '525
CCMB80 10mM 80mM 20mM 10% 7% pH 6.4 Invitrogen '464
TB 250mM 15mM 15mM 7% pH 5.7->6.2 Inoue90
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