Chemical transformation buffer comparison: Difference between revisions
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| Line 73: | Line 73: | ||
|- | |- | ||
|FSB | |FSB | ||
| | | | ||
|10mM | |10mM | ||
|10mM | |10mM | ||
| Line 80: | Line 80: | ||
|3mM | |3mM | ||
|8% | |8% | ||
| | |8% | ||
| | | | ||
| | | | ||
|pH 6.4->6.2 | |pH 6.4->6.2 | ||
| | |Stratagene '525 | ||
|- | |- | ||
|CCMB80 | |CCMB80 | ||
Revision as of 10:33, 2 May 2006
| Buffer | KCl | KCH3COO | CaCl2 | MnCl2 | RbCl | HACoCl3 | Glycerol | DMSO | Sucrose | Other | pH | Reference |
| SF | 100mM | 10mM | 50mM | 10% | pH 6.1 | Hanahan patent | ||||||
| RFT | 30mM | 10mM | 50mM | 100mM | 15% | pH 5.8 | Hanahan patent | |||||
| FSB | 100mM | 10mM | 45mM | 3mM | 10% w/v | pH 6.2 | Hanahan patent | |||||
| FSB | 100mM | 10mM | 10mM | 44mM | ? | 3mM | 10% | 2x 3.5% | 5% | pH 6.4->6.2 | Jessee patent | |
| FSB | 10mM | 10mM | 45mM | 3mM | 8% | 8% | pH 6.4->6.2 | Stratagene '525 | ||||
| CCMB80 | 10mM | 80mM | 20mM | 10% | 7% | pH 6.4 | Invitrogen '464 | |||||
| TB | 250mM | 15mM | 15mM | 7% | pH 5.7->6.2 | Inoue90 |
